Massarelli A C, Dainous F, Hoffmann D, Mykita S, Freysz L, Dreyfus H, Massarelli R
Neurochem Res. 1986 Jan;11(1):29-36. doi: 10.1007/BF00965162.
The uptake of radioactive ethanolamine has been studied in exclusively neuronal and glial cell cultures from dissociated cerebral hemispheres of chick embryos. Both cell types show saturable kinetics; neurons have an apparent Km of 6.7 microM, Vmax 41.4 pmol mg prot.-1 min-1 and glial cells a Km of 119.6 microM, Vmax 3,917 pmol mg prot-1 min-1. The lower affinity of the transport and the 100 fold increase in Vmax observed in glial cells correlated with a more important accumulation of free ethanolamine found in glial cells and with a higher degree of phosphorylation of ethanolamine. The uptake appeared to be temperature and Na+ ions dependent but was not affected by CN- or ouabain. Monomethyl-, dimethylethanolamine and choline were effective in inhibiting the uptake. Little or no effect was observed with serine, methionine, carnitine, alanine or glutamate.
已在来自鸡胚解离大脑半球的纯神经元和神经胶质细胞培养物中研究了放射性乙醇胺的摄取。两种细胞类型均表现出饱和动力学；神经元的表观 Km 为 6.7 μM，Vmax 为 41.4 pmol mg 蛋白-1 分钟-1，神经胶质细胞的 Km 为 119.6 μM，Vmax 为 3917 pmol mg 蛋白-1 分钟-1。在神经胶质细胞中观察到的转运亲和力较低以及 Vmax 增加 100 倍，与神经胶质细胞中游离乙醇胺的更重要积累以及乙醇胺的更高磷酸化程度相关。摄取似乎依赖于温度和 Na+ 离子，但不受 CN- 或哇巴因的影响。单甲基、二甲基乙醇胺和胆碱可有效抑制摄取。丝氨酸、蛋氨酸、肉碱、丙氨酸或谷氨酸几乎没有影响或没有影响。
