Elgendy Ashraf A, Tallima Hatem
Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt; Department of Immunology, New Kasr Al-Aini Teaching Hospital, Cairo, Egypt.
Department of Chemistry, School of Sciences and Engineering, The American University in Cairo, New Cairo, Cairo, Egypt.
Int Immunopharmacol. 2025 Jan 10;144:113661. doi: 10.1016/j.intimp.2024.113661. Epub 2024 Nov 26.
Despite its innumerable, invaluable and unique benefits to human development and welfare, consumption of the omega 6 polyunsaturated fatty acid, arachidonic acid (ARA) generates apprehension due to the association of its metabolites with allergy symptoms. Accordingly, it was deemed important to examine the impact of ARA supplementation on initiation and progress of peanut (PN)-induced allergy in mice of different MHC haplotypes.
Cohorts of BALB/c, C57BL/6, and outbred CD-1 mice were maintained two weeks before experimentation and until the end of the experiment on mouse food supplemented with equal amounts of milk powder containing 3 or 0 mg ARA/day/mouse, and then exposed to inhalation of 0 or 100 μg/mouse PN flour molecules twice for 4 weeks. Following intraperitoneal administration of PN extract proteins, control and PN-sensitized mice were assayed for behavioral, serum, and lung tissue biomarkers of anaphylaxis.
Peanut exposure essentially elicited the production of serum IgA, IgG1 and IgG2a specific antibodies and lung tissue extract reactive oxygen species. Adjuvant-free PN inhalation, especially when associated with ARA supplementation, displayed a significant (P < 0.05) counteractive impact on PN-induced responses in inbred and outbred mice.
Dietary arachidonic acid contributes to alleviation or suppression of PN-induced allergy biomarkers in BALB/c, C57BL/6 and CD-1 mice.
尽管ω-6多不饱和脂肪酸花生四烯酸（ARA）对人类发展和福祉具有无数、宝贵且独特的益处，但其代谢产物与过敏症状的关联引发了人们的担忧。因此，研究补充ARA对不同MHC单倍型小鼠花生（PN）诱导的过敏反应的起始和进展的影响被认为很重要。
在实验前两周及实验结束前，将BALB/c、C57BL/6和远交CD-1小鼠群体饲养在补充了等量含3或0毫克ARA/天/小鼠的奶粉的小鼠食物上，然后让其吸入0或100微克/小鼠的PN面粉分子，每周两次，持续4周。在腹腔注射PN提取物蛋白后，对对照小鼠和PN致敏小鼠进行过敏反应的行为、血清和肺组织生物标志物检测。
花生暴露基本上引发了血清IgA、IgG1和IgG2a特异性抗体的产生以及肺组织提取物活性氧的产生。无佐剂的PN吸入，特别是与补充ARA相关时，对近交和远交小鼠的PN诱导反应显示出显著（P<0.05）的对抗作用。
膳食花生四烯酸有助于减轻或抑制BALB/c、C57BL/6和CD-1小鼠中PN诱导的过敏生物标志物。
