Li Yajuan, Chen Liyi, Xiao Junfang, Feng Keyu, Zhang Xinheng, Chang Yung-Fu, Xie Qingmei
State Key Laboratory of Swine and Poultry Breeding Industry & Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China; Zhongshan Innovation Center of South China Agricultural University, Zhongshan 528400, PR China.
State Key Laboratory of Swine and Poultry Breeding Industry & Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China.
Vaccine. 2025 Jan 12;44:126532. doi: 10.1016/j.vaccine.2024.126532. Epub 2024 Nov 26.
Pasteurella multocida (P. multocida), a pathogenic bacterium known to induce duck cholera, stands as a significant contributor to bacterial diseases afflicting the duck industry, causing substantial annual economic losses on a global scale. In this study, the genes encoding the lipoproteins PlpE of P. multocida strain PMWSG-4 was cloned, inserted into the pBAD-ClyA vector, and the recombinant outer membrane vesicles (OMVs) fused with PlpE antigen of P. multocida was expressed by Escherichia coli (E. coli). Ducks immunized with OMV-PlpE had significantly (P < 0.001) increased production of antigen-specific antibodies. Moreover, at 28 days post-immunization, the expression of genes associated with immune response, including interleukin (IL)-2, IL-4, IL-10, and interferon (IFN)-γ in the spleen tissue of immunized ducks were significantly (P < 0.001) up-regulated compared to unimmunized ducks in the control group. And the active serum had significant bactericidal effects against the PMWSG-4 strain (P < 0.001). The protective efficacy of the vaccines was evaluated by leg muscle challenge with 20 LD50 doses of P. multocida, with the recombinant OMV-PlpE conferring 100 % protection. Histopathological examination and tissue bacterial load detection revealed that OMV-PlpE mitigated tissue damage and bacterial colonization to a statistically significant extent (P < 0.001). These findings serve as a valuable reference for the development of vaccines against P. multocida.
多杀性巴氏杆菌（P. multocida）是一种已知可引发鸭霍乱的病原菌，是困扰鸭产业的细菌性疾病的重要成因，在全球范围内每年造成巨大经济损失。在本研究中，克隆了多杀性巴氏杆菌菌株PMWSG - 4编码脂蛋白PlpE的基因，将其插入pBAD - ClyA载体，大肠杆菌（E. coli）表达了与多杀性巴氏杆菌PlpE抗原融合的重组外膜囊泡（OMV）。用OMV - PlpE免疫的鸭产生的抗原特异性抗体显著增加（P < 0.001）。此外，免疫后28天，与未免疫的对照组鸭相比，免疫鸭脾脏组织中与免疫反应相关的基因，包括白细胞介素（IL）-2、IL -4、IL -10和干扰素（IFN）-γ的表达显著上调（P < 0.001）。并且活性血清对PMWSG - 4菌株具有显著杀菌作用（P < 0.001）。通过腿部肌肉注射20个半数致死剂量（LD50）的多杀性巴氏杆菌评估疫苗的保护效力，重组OMV - PlpE提供了100%的保护。组织病理学检查和组织细菌载量检测表明，OMV - PlpE在统计学上显著减轻了组织损伤和细菌定植（P < 0.001）。这些发现为开发抗多杀性巴氏杆菌疫苗提供了有价值的参考。
