Fryer M W, Neering I R
Neurosci Lett. 1986 Feb 28;64(2):231-5. doi: 10.1016/0304-3940(86)90106-0.
Following injection of aequorin into small bundles of rat soleus and extensor digitorum longus muscles, simultaneous measurements of light (proportional to the intracellular free calcium ion concentration ([Ca2+]i)) and force were recorded during muscle twitches over a range of temperatures from 10 to 37 degrees C. The Arrhenius plots for rate of decline of [Ca2+]i following a twitch were linear and yielded the same activation enthalpy (Ea) for both muscle types, indicating that the same process controls Ca2+ uptake in both fast and slow muscles. Arrhenius plots of force decay rate constants showed a break around 20 to 25 degrees C with the Ea's above the break being similar to the Ea's for decline of [Ca2+]i and indicating a close relationship between rate of relaxation and decline of [CA2+]i above this temperature.
将水母发光蛋白注射到大鼠比目鱼肌和趾长伸肌的小肌束中后，在10至37摄氏度范围内的一系列温度下，于肌肉抽搐期间同步记录了光（与细胞内游离钙离子浓度[Ca2+]i成正比）和力量。抽搐后[Ca2+]i下降速率的阿伦尼乌斯图呈线性，且两种肌肉类型产生相同的活化焓（Ea），这表明相同的过程控制着快肌和慢肌中的Ca2+摄取。力量衰减速率常数的阿伦尼乌斯图在20至25摄氏度左右出现转折，转折温度以上的Ea与[Ca2+]i下降的Ea相似，表明在此温度以上，松弛速率与[Ca2+]i下降之间存在密切关系。
