Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.
Front Immunol. 2024 Nov 13;15:1481696. doi: 10.3389/fimmu.2024.1481696. eCollection 2024.
Dual-specificity phosphatases (DUSPs) are crucial regulators in many mammals, managing dephosphorylation and inactivation of mitogen-activated protein kinases (MAPKs) and playing essential roles in immune responses. However, their presence and functions in teleosts, like the yellow catfish (), remain unexplored.
In this study, eight genes ( and ) were identified in yellow catfish. We characterized their molecular features, conserved protein sequences, and chromosomal localization through genome-wide analyses, and we examined their expression patterns in immune responses.
Our findings reveal two conserved motifs, Leu-Phe-Leu-Gly and Ala-Tyr-Leu-Met, within the DSPc domain of DUSP proteins. The genes were mapped across seven chromosomes without evidence of duplication. Comparative analysis showed high conservation of Dusp genes across vertebrates, with evolutionary analysis suggesting as a potential intermediate form. transcripts were significantly upregulated in the kidney post- infection.
These results suggest the involvement of genes in the immune response of yellow catfish to bacterial pathogens, providing insights into their evolutionary significance and potential applications in aquaculture and molecular breeding.
双特异性磷酸酶（DUSPs）是许多哺乳动物中重要的调节因子，负责调节丝裂原活化蛋白激酶（MAPKs）的去磷酸化和失活，在免疫反应中发挥着重要作用。然而，它们在鱼类中的存在和功能，如黄颡鱼（），仍未被探索。
在本研究中，我们在黄颡鱼中鉴定了 8 个 基因（和）。我们通过全基因组分析对它们的分子特征、保守蛋白序列和染色体定位进行了表征，并研究了它们在免疫反应中的表达模式。
我们的研究结果揭示了 DUSP 蛋白的 DSPc 结构域内存在两个保守基序，亮氨酸-苯丙氨酸-亮氨酸-甘氨酸和丙氨酸-酪氨酸-亮氨酸-蛋氨酸。这些基因在没有证据表明存在重复的情况下被映射到 7 条染色体上。比较分析显示，Dusp 基因在脊椎动物中高度保守，进化分析表明 作为一种潜在的中间形式。感染后，肾脏中的 转录本显著上调。
这些结果表明 基因参与了黄颡鱼对细菌病原体的免疫反应，为它们的进化意义及其在水产养殖和分子育种中的潜在应用提供了新的见解。
