College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Gansu Agricultural University, Lanzhou, China.
Front Immunol. 2024 Nov 13;15:1454334. doi: 10.3389/fimmu.2024.1454334. eCollection 2024.
The gut-liver axis in ruminants can explain nutrient regulation, the gut-liver cycle, and immune function in ruminant biology through the gut microbe-gut metabolite-liver metabolite relationship. to investigate the effects of herbal feed additives on the gut-liver axis of Hu sheep. In this study, a broadly targeted UPLC-MS/MS metabolomics approach and 16s sequencing of gut microorganisms were used to detect, identify and quantify changes in ileal microorganisms, liver metabolites and ileal metabolites following the addition of Chinese herbal preparations. The addition of a 0.5% herbal feed additive increased ileal IgA, IgG and complement C3 levels. The addition of Chinese herbal preparations can increase the abundance of , , , at the portal level of the ileum, increase the metabolism of organic matter and its derivatives, bile acids, amino acids and their metabolites, coenzymes, and vitamins in the liver and ileum, enhance nutrient absorption and waste metabolism, accelerate liver metabolism, promote gut-liver circulation, and improve ileal and liver immunity. This study provides a theoretical basis for understanding the effects of herbal feed additives on the gut-liver axis in ruminants.
反刍动物的肠-肝轴可以通过肠道微生物-肠道代谢物-肝代谢物的关系来解释反刍动物生物学中的营养调节、肠-肝循环和免疫功能。本研究采用广靶 UPLC-MS/MS 代谢组学方法和肠道微生物 16s 测序技术，检测、鉴定和定量分析添加中草药制剂后回肠微生物、肝代谢物和回肠代谢物的变化。添加 0.5%的中草药饲料添加剂可提高回肠 IgA、IgG 和补体 C3 水平。添加中草药制剂可增加门静脉水平回肠中的、、、的丰度，增加肝脏和回肠中有机物及其衍生物、胆汁酸、氨基酸及其代谢物、辅酶和维生素的代谢，增强营养物质吸收和废物代谢，加速肝脏代谢，促进肠-肝循环，提高回肠和肝脏免疫力。本研究为了解中草药饲料添加剂对反刍动物肠-肝轴的影响提供了理论依据。
