Zhu Wenyuan G, Thomas Aaron Cq, Wilson Gary M, Hibbert Jamie E, Flynn Corey Gk, McGlory Chris, Jorgenson Kent W, Steinert Nathaniel D, Lin Kuan-Hung, MacInnis Martin J, Coon Joshua J, Phillips Stuart M, Hornberger Troy A
Department of Comparative Biosciences, University of Wisconsin-Madison.
School of Veterinary Medicine, University of Wisconsin-Madison.
Res Sq. 2024 Nov 12:rs.3.rs-4997138. doi: 10.21203/rs.3.rs-4997138/v1.
A human model of unilateral endurance versus resistance exercise, in conjunction with deep phosphoproteomic analyses, was used to identify exercise mode-specific phosphorylation events. Among the outcomes, a resistance exercise-specific cluster of events was identified, and a multitude of bioinformatic- and literature-based predictions suggested that this was mediated by prolonged activation of a pathway involving MKK3b/6, p38, MK2, and mTORC1. Follow-up studies in humans and mice provide consistent support for the predictions and also revealed that resistance exercise-induced signaling through MKK3b and the induction of protein synthesis are highly correlated events (R = 0.87). Moreover, genetic activation of MKK3b/6 in skeletal muscles was sufficient to induce signaling through the members of the resistance exercise-specific pathway, as well as an increase in protein synthesis and fiber size. Thus, we propose that we have identified some of the core components of a signaling pathway that drives the growth-promoting effects of resistance exercise.
通过建立单侧耐力与阻力运动的人体模型，并结合深度磷酸化蛋白质组分析，来确定运动模式特异性的磷酸化事件。在这些结果中，识别出了一组阻力运动特异性的事件，并且大量基于生物信息学和文献的预测表明，这是由涉及MKK3b/6、p38、MK2和mTORC1的信号通路的长期激活介导的。在人类和小鼠身上进行的后续研究为这些预测提供了一致的支持，并且还揭示了阻力运动通过MKK3b诱导的信号传导与蛋白质合成的诱导是高度相关的事件（R = 0.87）。此外，骨骼肌中MKK3b/6的基因激活足以诱导通过阻力运动特异性信号通路成员的信号传导，以及蛋白质合成和纤维大小的增加。因此，我们提出我们已经确定了驱动阻力运动促进生长作用的信号通路的一些核心成分。
