Bouvet Pierre, Bevilacqua Carlo, Ambekar Yogeshwari, Antonacci Giuseppe, Au Joshua, Caponi Silvia, Chagnon-Lessard Sophie, Czarske Juergen, Dehoux Thomas, Fioretto Daniele, Fu Yujian, Guck Jochen, Hamann Thorsten, Heinemann Dag, Jähnke Torsten, Jean-Ruel Hubert, Kabakova Irina, Koski Kristie, Koukourakis Nektarios, Krause David, La Cavera Salvatore, Landes Timm, Li Jinhao, Margueritat Jeremie, Mattarelli Maurizio, Monaghan Michael, Overby Darryl R, Perez-Cota Fernando, Pontecorvo Emanuele, Prevedel Robert, Ruocco Giancarlo, Sandercock John, Scarcelli Giuliano, Scarponi Filippo, Testi Claudia, Török Peter, Vovard Lucie, Weninger Wolfgang, Yakovlev Vladislav, Yun Seok-Hyun, Zhang Jitao, Palombo Francesca, Bilenca Alberto, Elsayad Kareem
Center for Anatomy and Cell Biology, Medical University of Vienna, Austria.
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Germany.
ArXiv. 2024 Nov 18:arXiv:2411.11712v1.
Brillouin Light Scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the sub-micron scale. Over the last decade it has seen increased applications in the life sciences, driven by the observed significance of mechanical properties in biological processes, the realization of more sensitive BLS spectrometers and its extension to an imaging modality. As with other spectroscopic techniques, BLS measurements not only detect signals characteristic of the investigated sample, but also of the experimental apparatus, and can be significantly affected by measurement conditions. The aim of this consensus statement is to improve the comparability of BLS studies by providing reporting recommendations for the measured parameters and detailing common artifacts. Given that most BLS studies of biological matter are still at proof-of-concept stages and use different--often self-built--spectrometers, a consensus statement is particularly timely to assure unified advancement.
布里渊光散射（BLS）光谱学是一种非侵入性、非接触式、无标记的光学技术，它能够在亚微米尺度上提供有关材料机械性能的信息。在过去十年中，由于机械性能在生物过程中的重要性被观察到、更灵敏的BLS光谱仪的实现以及其向成像模式的扩展，它在生命科学中的应用不断增加。与其他光谱技术一样，BLS测量不仅能检测被研究样品的特征信号，还能检测实验装置的信号，并且会受到测量条件的显著影响。本共识声明的目的是通过提供测量参数的报告建议并详细说明常见伪像，来提高BLS研究的可比性。鉴于大多数关于生物物质的BLS研究仍处于概念验证阶段，并且使用不同的——通常是自行构建的——光谱仪，一份共识声明对于确保统一进展尤为及时。
