Deng Zhang-Chao, Cao Ke-Xin, Huang Yu-Xuan, Peng Zhe, Zhao Ling, Yi Dan, Liu Meng, Sun Lv-Hui
State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Frontiers Science Center for Animal Breeding and Sustainable Production, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China.
Sci China Life Sci. 2025 Mar;68(3):836-845. doi: 10.1007/s11427-024-2735-8. Epub 2024 Nov 26.
Chicken gut microbiota plays an important role in maintaining their physiological health. However, the cultivability of chicken gut microbiota is not well understood, limiting the exploration of certain key gut bacteria in regulating intestinal health and nutritional metabolism. This study aimed to examine the cultivability of chicken cecal microbiota and to provide guidance for future chicken gut microbiota cultivation. A total of 58 different culture conditions were applied to culture broiler cecal microbiota, and the culture-dependent (CD; pooled colonies form each plate) and culture-independent (CI; broiler cecal contents) samples were collected for 16S rRNA gene sequencing and microbial analysis. The CD methods detected higher microbial richness (3,636 vs 2,331 OTUs) than CI methods, and the recovery rates of bacterial OTUs and genera reached 43.6% and 68.9%, respectively. The genera of Bacteroides (19.9%), Alistipes (11.0%) and Barnesiella (10.7%) were highly abundant detected by CI methods, however, there occupied a small proportion (<1.0%) of total cultured microbiota in CD methods. We then developed reference figures and tables showing optimal cultivation conditions for different gut bacteria taxa. Moreover, 81 different lactic acid bacteria strains covering 5 genera were isolated, and 15 strains had less than 97.0% similarity to known bacteria in the national center for biotechnology information (NCBI) online database. Overall, this study provides preliminary guidance in culturing specific gut microbiota from chickens, which will contribute to future studies to characterize the biological functions of key microbes in chicken nutritional metabolism and health.
鸡肠道微生物群在维持其生理健康方面发挥着重要作用。然而，人们对鸡肠道微生物群的可培养性了解不足，这限制了对某些关键肠道细菌在调节肠道健康和营养代谢方面的探索。本研究旨在检测鸡盲肠微生物群的可培养性，并为未来鸡肠道微生物群的培养提供指导。共应用58种不同的培养条件培养肉鸡盲肠微生物群，并收集基于培养法（CD；每块平板上的混合菌落）和非培养法（CI；肉鸡盲肠内容物）的样本进行16S rRNA基因测序和微生物分析。与CI方法相比，CD方法检测到的微生物丰富度更高（3636个OTU对2331个OTU），细菌OTU和属的回收率分别达到43.6%和68.9%。CI方法检测到的拟杆菌属（19.9%）、艾利斯菌属（11.0%）和巴恩斯菌属（10.7%）丰度很高，然而，在CD方法中，它们在总培养微生物群中所占比例较小（<1.0%）。然后，我们制定了参考图表，展示了不同肠道细菌类群的最佳培养条件。此外，还分离出了涵盖5个属的81株不同的乳酸菌菌株，其中15株与美国国立生物技术信息中心（NCBI）在线数据库中的已知细菌相似度低于97.0%。总体而言，本研究为从鸡中培养特定肠道微生物群提供了初步指导，这将有助于未来研究确定关键微生物在鸡营养代谢和健康中的生物学功能。
