Chen Zuchun, Zhou Tianyang, Chen Xiaotian, Huan Zhang, Huang Jianxuan, Lu Shulan, Zeng Manwen, Guo Yusong, Wang Zhongduo, Dong Zhongdian
Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China.
Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China.
Sci Total Environ. 2024 Dec 20;957:177745. doi: 10.1016/j.scitotenv.2024.177745. Epub 2024 Nov 27.
Bisphenol AF (BPAF), a substitute for bisphenol A (BPA), exhibits potent endocrine-disrupting properties that pose a serious health hazard to organisms. This study employed marine medaka as a model, subjecting them to different concentrations of BPAF (0.61, 6.65, and 91.88 μg/L) from the embryonic stage for a period of 160 days. Findings showed that 91.88 μg/L BPAF reduced survival rates and altered sex ratios. Furthermore, exposure to BPAF at all concentrations led to a significant increase in body length and weight. Behavioral analysis revealed that BPAF exposure impaired the swimming ability of the medaka. Histological changes included disrupted ovarian development, reduced sperm count, liver inflammation, and intestinal damage. Gene expression analysis revealed impacts on nervous system (e.g., gap43, itr, elavl3), HPG axis (e.g., gthα, erα, 3βhsd), and liver genes (e.g., chgl, vtg2). Additionally, BPAF altered the diversity and richness of gut microbes in marine medaka, leading to significant changes in specific bacterial species and intestinal functions. In conclusion, long-term BPAF exposure induced neurotoxicity, reproductive toxicity, and impaired digestive and immune systems in marine medaka, with sex-specific effects. These results provide further evidence of the potential hazards of BPAF as an environmental pollutant.
双酚AF（BPAF）作为双酚A（BPA）的替代品，具有强大的内分泌干扰特性，对生物体构成严重的健康危害。本研究以海洋青鳉为模型，使其从胚胎期开始暴露于不同浓度的BPAF（0.61、6.65和91.88μg/L）中，持续160天。研究结果表明，91.88μg/L的BPAF降低了存活率并改变了性别比例。此外，所有浓度的BPAF暴露均导致体长和体重显著增加。行为分析显示，BPAF暴露损害了青鳉的游泳能力。组织学变化包括卵巢发育受阻、精子数量减少、肝脏炎症和肠道损伤。基因表达分析揭示了对神经系统（如gap43、itr、elavl3）、HPG轴（如gthα、erα、3βhsd）和肝脏基因（如chgl、vtg2）的影响。此外，BPAF改变了海洋青鳉肠道微生物的多样性和丰富度，导致特定细菌种类和肠道功能发生显著变化。总之，长期暴露于BPAF会诱导海洋青鳉产生神经毒性、生殖毒性，并损害其消化和免疫系统，且具有性别特异性影响。这些结果进一步证明了BPAF作为一种环境污染物的潜在危害。
