Pan Chen, Liao Yifei, Shi Baojing, Zhang Manman, Zhou Yi, Wu Jiahao, Wu Hongxia, Qian Minjie, Bai Songling, Teng Yuanwen, Ni Junbei
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310058, PR China; Hainan Institute of Zhejiang University, Sanya, Hainan 572000, PR China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Hangzhou, Zhejiang 310058, PR China; The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, Zhejiang 310058, PR China.
National Key Laboratory for Tropical Crop Breeding, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524013, PR China.
Plant Physiol Biochem. 2025 Feb;219:109315. doi: 10.1016/j.plaphy.2024.109315. Epub 2024 Nov 22.
Blue light simultaneously enhances anthocyanin and carotenoid biosynthesis in mango (Mangifera indica L.) fruit peel and flesh, respectively, but the mechanism remains unclear. In this study, two blue light-triggered zinc-finger transcription factors, MiBBX24 and MiBBX27, that positively regulate anthocyanin and carotenoid biosynthesis in mango fruit were identified. Both MiBBXs transcriptionally activate the expression of MiMYB1, a positive regulator of anthocyanin biosynthesis. Furthermore, both MiBBXs also trigger the expression of a phytoene synthase gene (MiPSY), which is essential for carotenoid biosynthesis. Ectopic expression of MiBBX24 or MiBBX27 in Arabidopsis increased anthocyanin contents, and their positive effects on anthocyanin accumulation in mango peel were confirmed through transient overexpression and virus-induced silencing. Transient expression of MiBBX24 or MiBBX27 in tomato (Solanum lycopersicum) and mango fruit flesh increased the carotenoid content, while the virus-induced silencing of MiBBX24 or MiBBX27 in the mango fruit flesh decreased carotenoid accumulation. Overall, our study results reveal that MiBBX24 and MiBBX27 simultaneously promote the biosynthesis of anthocyanin and carotenoids biosynthesis in mango fruit peel and flesh under blue light, indicating that BBX-mediated dual effects on physiological functions contribute to mango fruit pigment accumulation. Furthermore, we herein shed new light on the simultaneous transcriptional regulatory effects of a single factor on the biosynthesis of different plant pigments.
蓝光可同时分别增强芒果（Mangifera indica L.）果皮和果肉中花青素和类胡萝卜素的生物合成，但具体机制尚不清楚。在本研究中，鉴定出了两个蓝光触发的锌指转录因子MiBBX24和MiBBX27，它们对芒果果实中花青素和类胡萝卜素的生物合成具有正向调控作用。这两个MiBBX转录激活花青素生物合成的正向调节因子MiMYB1的表达。此外，这两个MiBBX还能触发类胡萝卜素生物合成所必需的八氢番茄红素合成酶基因（MiPSY）的表达。MiBBX24或MiBBX27在拟南芥中的异位表达增加了花青素含量，通过瞬时过表达和病毒诱导沉默证实了它们对芒果果皮花青素积累的正向作用。MiBBX24或MiBBX27在番茄（Solanum lycopersicum）和芒果果肉中的瞬时表达增加了类胡萝卜素含量，而在芒果果肉中对MiBBX24或MiBBX27进行病毒诱导沉默则降低了类胡萝卜素的积累。总体而言，我们的研究结果表明，在蓝光条件下，MiBBX24和MiBBX27同时促进芒果果皮和果肉中花青素和类胡萝卜素的生物合成，这表明BBX介导的对生理功能的双重作用有助于芒果果实色素的积累。此外，我们在此揭示了单一因子对不同植物色素生物合成的同时转录调控作用。
