Zenelt Weronika, Krawczyk Krzysztof
Plant Disease Clinic and Bank of Pathogens, Institute of Plant Protection - National Research Institute, Władysława Węgorka 20 street, 60-318, Poznań, Poland.
Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Władysława Węgorka 20 street, 60-318, Poznań, Poland.
Microb Pathog. 2025 Jan;198:107158. doi: 10.1016/j.micpath.2024.107158. Epub 2024 Nov 26.
Sustainable agriculture is increasingly emphasized, focusing on microorganisms' role in maintaining soil fertility and inhibiting plant pathogens. Seeking novel sources of plant-beneficial bacteria, our study explores insects due to their established associations with plants and bacteria. The insect gut, hosting various bacteria, may hold microbes protecting against fungal infections, particularly plant pathogens. Traditional sources of plant growth-promoting bacteria are the rhizosphere and host plant tissues; however, insects serve as diverse bacterial reservoirs in the environment. This study aimed to identify insect-gut-derived bacteria with antifungal properties and cellulase enzyme production, predicting high plant tissue colonization abilities. Cellulase, crucial for breaking down cellulose, is essential for both industry and the environment. We sought to assess the potential of these bacteria as biocontrol agents against plant pathogenic fungi, with a focus on tomato plants. Bacterial isolates from insect bodies, including Lactococcus lactis, Pantoea ananatis, and Serratia liquefaciens, exhibited robust antifungal properties and cellulase activity. In vitro tests and glasshouse tests, confirmed their ability to inhibit the growth of plant-pathogenic fungi, indicating potential for biological control. Moreover, selected strains demonstrated high cellulase enzyme activity, vital for nutrient competition and rapid colonization of plant surfaces. The study introduces insect-gut-derived bacteria as promising biocontrol agents against plant pathogenic fungi. The identified strains, capable of inhibiting fungal growth and producing cellulase, offer sustainable alternatives to synthetic fungicides for protecting tomato plants. The findings advance agricultural practices by harnessing insect-associated bacteria, contributing to eco-friendly and efficient pest management strategies in modern agriculture.
可持续农业越来越受到重视，重点关注微生物在维持土壤肥力和抑制植物病原体方面的作用。为了寻找新型有益植物的细菌来源，我们的研究探索了昆虫，因为它们与植物和细菌之间存在既定的关联。昆虫肠道中存在各种细菌，可能含有能抵御真菌感染，特别是植物病原体的微生物。促进植物生长的细菌的传统来源是根际和寄主植物组织；然而，昆虫在环境中是多种多样的细菌储存库。本研究旨在鉴定具有抗真菌特性和纤维素酶产生能力、预测具有高植物组织定殖能力的昆虫肠道来源细菌。纤维素酶对于分解纤维素至关重要，对工业和环境都必不可少。我们试图评估这些细菌作为针对植物病原真菌的生物防治剂的潜力，重点是番茄植株。从昆虫体内分离出的细菌，包括乳酸乳球菌、菠萝泛菌和液化沙雷氏菌，表现出强大的抗真菌特性和纤维素酶活性。体外试验和温室试验证实了它们抑制植物病原真菌生长的能力，表明其具有生物防治潜力。此外，所选菌株表现出高纤维素酶活性，这对于营养竞争和植物表面的快速定殖至关重要。该研究引入了昆虫肠道来源细菌作为对抗植物病原真菌的有前景的生物防治剂。所鉴定的菌株能够抑制真菌生长并产生纤维素酶，为保护番茄植株提供了替代合成杀菌剂的可持续选择。这些发现通过利用与昆虫相关的细菌推进了农业实践，为现代农业中生态友好和高效的害虫管理策略做出了贡献。
