Prasannakumar S P, Gowtham H G, Hariprasad P, Shivaprasad K, Niranjana S R
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India.
Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India.
Lett Appl Microbiol. 2015 Nov;61(5):460-8. doi: 10.1111/lam.12479. Epub 2015 Sep 29.
The bacterial strain designated as WGR-UOM-BT1 isolated from rhizosphere of Rauwolfia serpentina exhibited broad-spectrum antifungal activity and also improved early plant growth. Based on morphological, biochemical and 16S rRNA gene sequence analyses, the strain BT1 was identified as Delftia tsuruhatensis (KF727978). Under in vitro conditions, the strain BT1 suppressed the growth of wide range of fungal phytopathogens. Purified antimicrobial metabolite from the strain BT1 was identified as nitrogen-containing heterocyclic compound, 'amino(5-(4-methoxyphenyl)-2-methyl-2-(thiophen-2-yl)-2,3-dihydrofuran-3-yl)methanol' (AMTM), with molecular mass of 340•40 and molecular formula of C17 H19 NO3 S. The strain BT1 was positive for rhizosphere colonization (tomato), IAA production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity and phosphate solubilization. Under laboratory and greenhouse conditions, the strain BT1 promoted plant growth and suppressed foliar and root fungal pathogens of tomato. Therefore, antimicrobial and disease protection properties of strain BT1 could serve as an effective biological control candidate against devastating fungal pathogens of vegetable plants. Besides, the production of IAA, P solubilization and ACC deaminase activity enhance its potential as a biofertilizer and may stabilize the plant performance under fluctuating environmental conditions.
In this study, we reported that Delftia tsuruhatensis WGR-UOM-BT1 strain has the plant growth promotion activities such as rhizosphere colonization (tomato), IAA production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity and phosphate solubilization. This bacterial strain was found producing an antimicrobial nitrogen-containing heterocyclic compound identified as 'amino(5-(4-methoxyphenyl)-2-methyl-2-(thiophen-2-yl)-2,3-dihydrofuran-3-yl)methanol' [C17 H19 NO3 S] (AMTM), which is new to the bacterial world.
从蛇根木根际分离出的名为WGR-UOM-BT1的细菌菌株表现出广谱抗真菌活性，还能促进植物早期生长。基于形态学、生化和16S rRNA基因序列分析，菌株BT1被鉴定为鹤见德尔福特菌（KF727978）。在体外条件下，菌株BT1抑制了多种真菌植物病原体的生长。从菌株BT1中纯化出的抗菌代谢产物被鉴定为含氮杂环化合物“氨基（5-（4-甲氧基苯基）-2-甲基-2-（噻吩-２-基）-2,3-二氢呋喃-3-基）甲醇”（AMTM），分子量为340.40，分子式为C17 H19 NO3 S。菌株BT1在根际定殖（番茄）、吲哚乙酸（IAA）产生、1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性和磷酸盐溶解方面呈阳性。在实验室和温室条件下，菌株BT1促进了植物生长，并抑制了番茄的叶部和根部真菌病原体。因此，菌株BT1的抗菌和病害防治特性可作为对抗蔬菜植物毁灭性真菌病原体的有效生物防治候选物。此外，IAA的产生、磷溶解和ACC脱氨酶活性增强了其作为生物肥料的潜力，并可能在波动的环境条件下稳定植物性能。
在本研究中，我们报道了鹤见德尔福特菌WGR-UOM-BT1菌株具有促进植物生长的活性，如根际定殖（番茄）、IAA产生、ACC脱氨酶活性和磷酸盐溶解。发现该细菌菌株产生一种抗菌含氮杂环化合物，鉴定为“氨基（5-（4-甲氧基苯基）-2-甲基-2-（噻吩-２-基）-2,3-二氢呋喃-3-基）甲醇”[C17 H19 NO3 S]（AMTM），这在细菌界是新发现的。
