Zhou Ruiyun, Wu Xinchen, Xue Shanshan, Yin Limei, Gao Shipeng, Zhang Yang, Wang Chen, Wang Yansong, El-Seedi Hesham R, Zou Xiaobo, Guo Zhiming
China Light Industry Key Laboratory of Food Intelligent Detection & Processing, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Focusight Technology (Jiangsu) Co., LTD, Changzhou 213100, China.
China Light Industry Key Laboratory of Food Intelligent Detection & Processing, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Food Chem. 2025 Feb 28;466:142200. doi: 10.1016/j.foodchem.2024.142200. Epub 2024 Nov 23.
Patulin (PAT), a major contaminant in apples, poses a considerable food safety risk, necessitating a rapid, sensitive and reliable detection method. This study developed a novel magnetic metal-organic frameworks (MOFs)-based ratiometric surface-enhanced Raman scattering (SERS) aptasensor. The sensor consists of magnetic MOFs loaded with 4-Mercaptobenzoic acid (4-MBA) internal labeled Au@Ag as the SERS substrate, and gold nanorods (AuNRs) modified with Rhodamine 6G and aptamers as capture probes. This strategy enhances sensitivity through magnetic separation and abundant SERS hotspots provided by the magnetic MOF nanocomposites. The SERS intensity ratio showed a negative correlation with PAT concentrations from 0.01 to 100 ng/mL, with a LOD of 0.0465 ng/mL. Moreover, the aptasensor achieved 95.90 % ∼ 105.83 % recoveries in apple samples, indicating high accuracy and anti-interference capability. The excellent sensing performance demonstrates great potential of the SERS nanosensor for mycotoxin detection in actual food matrices.
展青霉素（PAT）是苹果中的主要污染物，会带来相当大的食品安全风险，因此需要一种快速、灵敏且可靠的检测方法。本研究开发了一种基于磁性金属有机框架（MOFs）的新型比率表面增强拉曼散射（SERS）适配体传感器。该传感器由负载有4-巯基苯甲酸（4-MBA）内部标记的Au@Ag作为SERS基底的磁性MOFs，以及用罗丹明6G和适配体修饰的金纳米棒（AuNRs）作为捕获探针组成。这种策略通过磁性分离以及磁性MOF纳米复合材料提供的丰富SERS热点来提高灵敏度。SERS强度比与0.01至100 ng/mL的PAT浓度呈负相关，检测限为0.0465 ng/mL。此外，该适配体传感器在苹果样品中的回收率为95.90%至105.83%，表明其具有高准确性和抗干扰能力。出色的传感性能证明了SERS纳米传感器在实际食品基质中检测霉菌毒素的巨大潜力。
