Uhl G R, Reppert S M
Science. 1986 Apr 18;232(4748):390-3. doi: 10.1126/science.3961487.
In situ hybridization of an oligonucleotide probe complementary to vasopressin messenger RNA (mRNA) in sections from normal or Brattleboro rat hypothalami revealed hybridization densities in each of three vasopressin-rich nuclei: the supraoptic, paraventricular, and suprachiasmatic. When entrained to a daily light-dark cycle, each rat strain displayed diurnal variation in hybridizable mRNA in the suprachiasmatic, but not in the supraoptic or paraventricular nuclei. The higher values for suprachiasmatic mRNA in the morning correlate well with previously elucidated morning increases in vasopressin immunoreactivity in the cerebrospinal fluid. These results support the utility of in situ hybridization techniques for elucidating physiological influences on regional peptidergic function, are consistent with a prominent role for vasopressinergic suprachiasmatic neurons in generating the cerebrospinal fluid vasopressin rhythm, and suggest that regulation of this mRNA rhythm is not dependent on release of intact peptide.
在正常或布拉德福德大鼠下丘脑切片中，用与抗利尿激素信使核糖核酸（mRNA）互补的寡核苷酸探针进行原位杂交，结果显示，在三个富含抗利尿激素的核团（视上核、室旁核和视交叉上核）中均有杂交密度。当大鼠适应每日明暗周期时，每个大鼠品系的视交叉上核中可杂交mRNA呈现昼夜变化，但视上核或室旁核中则没有。视交叉上核mRNA在早晨的较高值与先前阐明的脑脊液中抗利尿激素免疫反应性早晨升高密切相关。这些结果支持原位杂交技术在阐明对局部肽能功能的生理影响方面的实用性，与血管加压素能视交叉上核神经元在产生脑脊液血管加压素节律中起重要作用相一致，并表明这种mRNA节律的调节不依赖于完整肽的释放。
