A rapid protocol for distinguishing the quality of Sanshengyin and identifying potential markers by the "three-in-one" fingerprint profiles with antioxidant activity.

It is imperative to establish a standardized model for the quality control of traditional prescriptions, exemplified by Sanshengyin (SSY). This study introduces a multi-component and multi-level "three-in-one" fingerprint quality evaluation system designed to predict the comprehensive quality of traditional Chinese medicine (TCM). Initially, a five-wavelength high-performance liquid chromatography (HPLC) fusion spectrum was developed to enhance the stability of SSY within the chromatographic system. Additionally, a quantification method was employed to streamline the calculation and visualization processes of ultraviolet (UV) and infrared (IR) spectral systems. Through this approach, chromatographic and spectral fingerprints were amalgamated into a holistic analytical strategy. The average linear quantitative fingerprint method (ALQFM) was employed to swiftly differentiate the grades of 17 sample batches. Additionally, six quality markers were identified for content determination. Correlation analysis between the total content of these quality markers and macroscopic quantitative similarity was conducted to assess the accuracy of ALQFM in evaluating the quality of TCM represented by SSY. Finally, the binary correlation analysis model was employed to investigate the correlation between antioxidant activity and fingerprint profiles, facilitating the prediction of potential bioactive compounds in SSY. This research offers a straightforward, cost-effective, and holistic approach for assessing the quality consistency of TCM. The method is recommended for adoption by medical institutions and enterprises to enhance the standardization of TCM quality control.