评价来自糖尿病足感染和尿路感染分离的变形杆菌菌株的毒力基因。
Evaluation of virulence genes in Proteus strains isolated from diabetic foot infections and urinary tract infections.
机构信息
Recombinant DNA and Recombinant Protein Research Center (REDPROM), Adnan Menderes University, Aydın, Türkiye.
Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Adnan Menderes University, Aydın, Türkiye.
出版信息
J Infect Dev Ctries. 2024 Oct 31;18(10):1559-1565. doi: 10.3855/jidc.18928.
INTRODUCTION
Proteus species are frequently isolated from urinary tract infections (UTIs) and diabetic foot infections (DFIs). We aimed to evaluate the presence of virulence genes in P. mirabilis and P. vulgaris strains isolated from DFI and UTI.
METHODOLOGY
A total of 78 Proteus isolates (57 P. mirabilis and 21 P. vulgaris) collected from patients were studied. The isolates were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. The presence of virulence-associated genes (hlyA, mrpA, atfA, pmfA, hmpA, ptaA, ureA, ureC, zapA, ireA, rsbA, flaA, and ucaA) was evaluated by polymerase chain reaction (PCR).
RESULTS
46 isolates were obtained from wound cultures, and 32 were obtained from the midstream urine cultures. All virulence genes, except hlyA, were detected in the study. ureA was the most detected gene in both UTI (100%) and DFI isolates (84.8%). The distributions of ureC, flaA, hpmA, ireA, rsbA, pmfA, zapA, ucaA, ptaA, atfA, and mrpA genes in DFI and UTI isolates were as follows: 82.6% and 96.9%, 71.7% and 93.8%, 69.6% and 93.8%, 69.6% and 96.9%, 69.6% and 96.9, 67.4% and 81.3%, 65.2% and 43.8%, 54.3 and 71.9%, 34.8% and 96.9%, 26.1 and 93.8%, and 23.9% and 46.9%, respectively.
CONCLUSIONS
We demonstrated that P. mirabilis and P. vulgaris express a variety of virulence genes related to pathogenicity. All virulence genes were found to be more frequent in UTI isolates except zapA. There is limited data on the virulence factors of Proteus species in DFIs. Further studies are needed to investigate virulence genes in wound isolates.
简介
变形菌属（Proteus）物种经常从尿路感染（UTI）和糖尿病足感染（DFI）中分离出来。我们旨在评估从 DFI 和 UTI 分离的奇异变形菌（P. mirabilis）和普通变形菌（P. vulgaris）菌株中存在的毒力基因。
方法
共研究了 78 株从患者中分离出的变形菌属（57 株奇异变形菌和 21 株普通变形菌）。使用基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱仪对分离株进行鉴定。通过聚合酶链反应（PCR）评估与毒力相关的基因（hlyA、mrpA、atfA、pmfA、hmpA、ptaA、ureA、ureC、zapA、ireA、rsbA、flaA 和 ucaA）的存在情况。
结果
46 株从伤口培养物中分离，32 株从中段尿培养物中分离。除 hlyA 外，研究中均检测到所有毒力基因。ureA 是 UTI（100%）和 DFI 分离株（84.8%）中检测到的最常见基因。ureC、flaA、hpmA、ireA、rsbA、pmfA、zapA、ucaA、ptaA、atfA 和 mrpA 基因在 DFI 和 UTI 分离株中的分布如下：82.6%和 96.9%、71.7%和 93.8%、69.6%和 93.8%、69.6%和 96.9%、69.6%和 96.9%、67.4%和 81.3%、65.2%和 43.8%、54.3%和 71.9%、34.8%和 96.9%、26.1%和 93.8%以及 23.9%和 46.9%。
结论
我们证明奇异变形菌和普通变形菌表达多种与致病性相关的毒力基因。除 zapA 外，所有毒力基因在 UTI 分离株中更为常见。关于变形菌属在 DFI 中的毒力因子的数据有限。需要进一步研究来调查伤口分离株中的毒力基因。
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