Bedir Esra, Ay Hilal, Kotil Enes Seyfullah, Ozbek Tulin
Yildiz Technical University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkey.
Bogazici University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkey.
Microb Pathog. 2025 Feb;199:107142. doi: 10.1016/j.micpath.2024.107142. Epub 2024 Nov 29.
During a study investigating possible probiotics from chicken gut microbiota, strains C1-4 and C2-3 were isolated and identified as members of the genus Ligilactobacillus. The strains formed a well-supported cluster with Ligilactobacillus salivarius and Ligilactobacillus hayatikensis in phylogenetic trees. Their genomes, sized 1.8 Mb with G + C content of 32 %, were related to "Candidatus Avacholeplasma faecigallinarum" with a dDDH level of 95.4 %, indicating the strains were the first culturable members of the uncultured taxon. Furthermore, a dDDH value of 78.9 % with L. salivarius DSM 20555 suggested that the strains may represent a novel subspecies of L. salivarius. The functional analysis of the genomes revealed that the strains harbour genes associated with probiotic traits, including lactate utilization, acetoin and butanediol metabolism, pH homeostasis and exopolysaccharide biosynthesis. The genome annotation for the secondary metabolite biosynthesis gene clusters showed that the strains have a type III polyketide gene cluster and a bacteriocin immunity protein gene. The strains exhibited phenotypic features compatible with their potential use as probiotics, such as tolerance to low pH and NaCl, ability to achieve high auto-aggregation, and hydrophobicity properties. In addition, the strains exhibited strong antibacterial activity against pathogenic MRSA (Meticillin-resistant Staphylococcus aureus ATCC 67101), S. aureus ATCC 25923, Listeria monocytogenes MBG16, and VRSA (Vancomycin-resistant Staphylococcus aureus MBG89), while showing moderate activity against Salmonella Typhimurium MBG15, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13833, and Pseudomonas aeruginosa ATCC 27853. The cell-free supernatant of the strains notably affected Lis. monocytogenes and S. aureus, possibly due to possible bacteriocin production. In conclusion, the strains isolated from chicken gut microbiota have a high potential to be used as probiotics in agriculture and medicine.
在一项对鸡肠道微生物群中可能存在的益生菌进行研究的过程中，分离出了C1-4和C2-3菌株，并鉴定它们为Ligilactobacillus属的成员。在系统发育树中，这些菌株与唾液Ligilactobacillus和哈亚蒂Ligilactobacillus形成了一个支持度良好的聚类。它们的基因组大小为1.8 Mb，G+C含量为32%，与“粪鸡嗜胆甾原体（Candidatus Avacholeplasma faecigallinarum）”相关，dDDH水平为95.4%，这表明这些菌株是未培养分类单元的首批可培养成员。此外，与唾液Ligilactobacillus DSM 20555的dDDH值为78.9%，表明这些菌株可能代表唾液Ligilactobacillus的一个新亚种。基因组的功能分析表明，这些菌株含有与益生菌特性相关的基因，包括乳酸利用、3-羟基丁酮和丁二醇代谢、pH稳态和胞外多糖生物合成。次生代谢物生物合成基因簇的基因组注释表明，这些菌株有一个III型聚酮基因簇和一个细菌素免疫蛋白基因。这些菌株表现出与其作为益生菌潜在用途相符的表型特征，如对低pH和NaCl的耐受性、实现高自聚集的能力以及疏水性。此外，这些菌株对致病性耐甲氧西林金黄色葡萄球菌（Meticillin-resistant Staphylococcus aureus ATCC 67101）、金黄色葡萄球菌ATCC 25923、单核细胞增生李斯特菌MBG16和耐万古霉素金黄色葡萄球菌（Vancomycin-resistant Staphylococcus aureus MBG89）表现出较强的抗菌活性，而对鼠伤寒沙门氏菌MBG15、大肠杆菌ATCC
