Su Chengxin, Liu Xiaoling, Zhang Ke, Jiang Bing, Hu Jiashuai, Li Mei, Cheng Lin, Luo Hongbing, Xie Wanchen, Liu Cheng, Fan Liangqian, Chen Wei, Zhang Xiaohong
Department of Municipal Engineering, College of Civil Engineering, Sichuan Agricultural University, Chengdu, 611830, China.
Department of Information Engineering, Sichuan Water Conservancy Vocational College, Chengdu, 611231, China.
Anal Biochem. 2025 Mar;698:115730. doi: 10.1016/j.ab.2024.115730. Epub 2024 Nov 30.
Given the adverse effects of tonalide (AHTN) on aquatic organisms and humans, coupled with the limitations of current detection methods, which are time-consuming, require expensive equipment and complicated sample preparation procedures, there is a clear need to develop a new technique for detecting AHTN that is highly sensitive, rapid, cost-effective and efficient. In this study, a new simple electrochemical sensor for the determination of AHTN in aqueous environments was developed for the first time through the in-situ polymerization of an AHTN-imprinted polymer on the surface of a graphene (G)-modified carbon electrode (GCE). Following a series of comparative tests, including cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), the novel AHTN molecularly imprinted sensor (AHTN-MIP/G/GCE) has been demonstrated to be an effective tool for monitoring AHTN. The results demonstrate that the linear detection range of the current response of the AHTN-MIP/G/GCE electrode to AHTN was 0.01 μM-4 μM (i.e., 2.584 μg/L-1033.6 μg/L), with a detection limit of 2.3 × 10⁻⁹ M (i.e., 594.32 ng/L), following the optimization of the experimental conditions. Furthermore, the new sensor was successfully employed for the detection of AHTN in water samples, with recoveries of 97.1%-108.2 % with the added standards. Consequently, the new electrochemical sensor demonstrated good stability and acceptable reproducibility. This study provides a new method for the future detection of AHTN in the aqueous environment.
鉴于 tonalide（AHTN）对水生生物和人类的不利影响，再加上当前检测方法存在耗时、需要昂贵设备和复杂样品制备程序等局限性，显然有必要开发一种新的检测 AHTN 的技术，该技术要具有高灵敏度、快速、经济高效的特点。在本研究中，首次通过在石墨烯（G）修饰的碳电极（GCE）表面原位聚合 AHTN 印迹聚合物，开发了一种用于测定水环境中 AHTN 的新型简单电化学传感器。经过一系列对比测试，包括循环伏安法（CV）、电化学阻抗谱（EIS）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM），新型 AHTN 分子印迹传感器（AHTN-MIP/G/GCE）已被证明是监测 AHTN 的有效工具。结果表明，在优化实验条件后，AHTN-MIP/G/GCE 电极对 AHTN 的电流响应线性检测范围为 0.01 μM - 4 μM（即 2.584 μg/L - 1033.6 μg/L），检测限为 2.3×10⁻⁹ M（即 594.32 ng/L）。此外，该新型传感器成功用于水样中 AHTN 的检测，加标回收率为 97.1% - 108.2%。因此，新型电化学传感器表现出良好的稳定性和可接受的重现性。本研究为未来水环境中 AHTN 的检测提供了一种新方法。
