Laboratory for Agricultural Productions Improvement, Biotechnology and Environment (LAPABE), Faculty of Sciences, University Mohammed First.
J Oleo Sci. 2024;73(12):1529-1540. doi: 10.5650/jos.ess24034.
Global distribution of prickly pear spans worldwide, with limited cladode exploitation, particularly in Mediterranean regions, excluding American continent nations. Our research reports on three species found in eastern Morocco, including Opuntia ficus indica and the two newly introduced species Opuntia robusta and Opuntia dillenii. The study aims to evaluate the total phenolic content, the amounts of various phenolic compounds classes, and the antioxidant activity across a spectrum of biochemical measurements including the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant potential (FRAP), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and total antioxidant capacity assay (TAC). The results showed that O. robusta exhibited the highest total phenolic content (5061.70 mg GAE/100 g) which enhances the significance of this species in terms of nutrition. Differences were also observed in different stage of cladodes maturity, demonstrating that the age factor affects the polyphenol contents. Moreover, O. robusta displays the highest amount of total phenolic compounds (11430.35 μg/g) either for flavonoids or phenolic acids. Furthermore, the oldest cladodes show more higher phenolic compounds (22998.62 μg/g) compared to the youngest one's (20438.54 μg/g). Ten phenolic compounds were identified, in which isorhamnetin represent the most abundant flavonoid (6747.85 μg/g), while the chlorogenic acid was determined as the major phenolic acid (2771.69 μg/g) followed by sinapic acid (2047.64 μg/g). The DPPH assay indicates that O. ficus-indica possessed the higher hydrophilic antioxidants able to reduction the 2,2-diphenyl-1-picrylhydrazylradical free radical. While O. robusta exhibited a greater quantity of antioxidants, which effectively reduced the Fe complex of ferric ions (TPTZ) . In contrast, old cladodes of O. robusta and O. dillenii demonstrate the higher ability to scavenge the 2,20-azinobis (3-ethylbenzthiazolin-6-sulfonic acid) or ABTS .
仙人掌在全球范围内分布广泛，但其肉质茎的利用有限，特别是在地中海地区，不包括美洲国家。我们的研究报告了在摩洛哥东部发现的三种仙人掌，包括仙人球和两种新引入的物种，即仙人柱和仙人棒。该研究旨在评估总酚含量、各种酚类化合物的含量以及抗氧化活性，通过一系列生化测量，包括 2,2-二苯基-1-苦基肼（DPPH）、铁还原抗氧化能力（FRAP）、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和总抗氧化能力测定（TAC）。结果表明，仙人柱表现出最高的总酚含量（5061.70 mg GAE/100 g），这增加了该物种在营养方面的重要性。不同成熟阶段的肉质茎也存在差异，表明年龄因素影响多酚含量。此外，仙人柱表现出最高的总酚类化合物含量（11430.35 μg/g），无论是类黄酮还是酚酸。此外，最老的肉质茎比最年轻的肉质茎含有更多的酚类化合物（22998.62 μg/g）。鉴定出了 10 种酚类化合物，其中异鼠李素是最丰富的类黄酮（6747.85 μg/g），绿原酸是主要的酚酸（2771.69 μg/g），其次是芥子酸（2047.64 μg/g）。DPPH 测定表明，仙人球具有较高的亲水性抗氧化剂，能够还原 2,2-二苯基-1-苦基肼自由基。而仙人柱则表现出更多的抗氧化剂，能够有效地还原三价铁离子（TPTZ）的铁复合物。相比之下，仙人柱和仙人棒的老肉质茎表现出更高的清除 2,20-联氮-双（3-乙基苯并噻唑啉-6-磺酸）或 ABTS 的能力。
