Claiborne Alex, Jevtovic Filip, Zheng Donghai, Strom Cody, Wisseman Breanna, McDonald Samantha, Newton Edward, Mouro Steven, DeVente James, Houmard Joseph A, Broskey Nicholas T, May Linda E
Department of Kinesiology, East Carolina University (ECU), Greenville, North Carolina, USA.
Human Performance Laboratory, ECU, Greenville, North Carolina, USA.
Physiol Rep. 2024 Dec;12(23):e70145. doi: 10.14814/phy2.70145.
Non-pharmaceutical interventions are needed to target the growing intergenerational cycle of obesity. We aimed to determine whether in utero exposure to different exercise doses during pregnancy directly reduces infant cellular and whole-body adiposity. Pregnant women completed ~24 weeks of supervised exercise training; for standardization of exercise analysis (frequency, intensity, time, and volume-FIT-V), metrics were assessed from 16 to 36 weeks. Mesenchymal stem cells (MSC) collected from the umbilical cord at delivery underwent 21 days of adipogenic differentiation, then Oil Red O staining for lipid content. Infant body composition was measured at 1 month of age. ANCOVA and Pearson correlations determined the influence of prenatal exercise on infant adiposity. Exercise decreased infant MSC lipid content (p = 0.01) and body fat percentage (p = 0.009) irrespective of dose. Total exercise volume throughout pregnancy was negatively correlated with infant body fat % (R = 0.31, p = 0.02) due to lower subscapular skinfolds (R = 0.30, p = 0.02), while weekly exercise duration influenced adipogenic MSC lipid accumulation (R = -0.23, p = 0.03) and BF% (R = -0.15, p = 0.01). In utero exposure to exercise reduced cellular and whole-body infant adiposity in a dose-dependent manner.
需要采取非药物干预措施来应对日益严重的肥胖代际循环问题。我们旨在确定孕期子宫内暴露于不同运动剂量是否能直接降低婴儿的细胞和全身脂肪含量。孕妇完成了约24周的有监督的运动训练；为了标准化运动分析（频率、强度、时间和运动量-FIT-V），在孕16至36周期间评估各项指标。分娩时从脐带采集的间充质干细胞（MSC）进行了21天的成脂分化，然后用 Oil Red O 染色检测脂质含量。在婴儿1月龄时测量其身体成分。协方差分析和皮尔逊相关性分析确定了产前运动对婴儿肥胖的影响。无论运动剂量如何，运动均降低了婴儿MSC的脂质含量（p = 0.01）和体脂百分比（p = 0.009）。由于肩胛下皮褶厚度降低（R = 0.30，p = 0.02），整个孕期的总运动量与婴儿体脂百分比呈负相关（R = 0.31，p = 0.02），而每周运动时长影响成脂MSC的脂质积累（R = -0.23，p = 0.03）和体脂百分比（R = -0.15，p = 0.01）。子宫内暴露于运动以剂量依赖的方式降低了婴儿的细胞和全身脂肪含量。
