Maitani T, Watahiki A, Suzuki K T
Toxicol Appl Pharmacol. 1986 Apr;83(2):211-7. doi: 10.1016/0041-008x(86)90298-x.
The amount of induced hepatic metallothionein (MT) and the alterations of calcium (Ca) and lead (Pb) concentrations in plasma, liver, kidney, and spleen were compared in male mice after iv, ip, and sc injections of lead acetate at a dose of 30 mg Pb/kg body wt. The amount of hepatic MT at 1 day was in the order of ip greater than iv greater than sc injection approximately 0, despite the hepatic Pb concentration in the order of iv greater than ip greater than sc injection. Heat-stable Pb-binding MT was not detected following any injection route. After the iv injection, a transitory hypercalcemia with hyperphosphatemia was observed. As for the tissue Pb concentration after the iv and ip injections, liver and spleen showed a high concentration, while kidney concentration was relatively low. The high tissue Pb was accompanied by an increase of tissue Ca in most cases. Only 10 to 15% of the total Pb accumulated in the liver at 1 day was recovered from the supernatant fraction after ultracentrifugation. The increase of hepatic Ca was ascribed to that in the sediment fraction. After the sc injection, the tissue Pb concentration was very low and no alterations were observed in tissue Ca concentrations.
给雄性小鼠静脉注射、腹腔注射和皮下注射剂量为30mg铅/千克体重的醋酸铅后，比较了诱导性肝金属硫蛋白（MT）的含量以及血浆、肝脏、肾脏和脾脏中钙（Ca）和铅（Pb）浓度的变化。尽管肝脏中铅浓度的顺序为静脉注射＞腹腔注射＞皮下注射，但第1天时肝脏MT的含量顺序为腹腔注射＞静脉注射＞皮下注射，皮下注射时MT含量几乎为0。任何注射途径后均未检测到热稳定的铅结合MT。静脉注射后，观察到短暂的高钙血症伴高磷血症。静脉注射和腹腔注射后，肝脏和脾脏的组织铅浓度较高，而肾脏中的浓度相对较低。在大多数情况下，组织中高浓度的铅伴随着组织钙的增加。超离心后，第1天时肝脏中积累的总铅只有10%至15%从上清液部分中回收。肝脏中钙的增加归因于沉淀部分中的增加。皮下注射后，组织铅浓度非常低，且未观察到组织钙浓度的变化。
