Xiao Kai-Zhen, Liao Gui, Huang Guang-Yu, Huang Yun-Long, Gu Rong-He
Department of Orthopedic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning 530022, Guangxi Zhuang Autonomous Region, China.
Graduate School of Guangxi Medical University, Guangxi Medical University, Nanning 530022, Guangxi Zhuang Autonomous Region, China.
World J Stem Cells. 2024 Nov 26;16(11):944-955. doi: 10.4252/wjsc.v16.i11.944.
We investigated the efficacy of intra-articular injection of human umbilical cord mesenchymal stem cells (hUC-MSCs) for the treatment of osteoarthritis (OA) progression in the knee joint. Although many experimental studies of hUC-MSCs have been published, these studies have mainly used fetal bovine serum-containing cultures of hUC-MSCs; serum-free cultures generally avoid the shortcomings of serum-containing cultures and are not subject to ethical limitations, have a wide range of prospects for clinical application, and provide a basis or animal experimentation for clinical experiments.
To study the therapeutic effects of serum-free hUC-MSCs (N-hUCMSCs) in a mouse model of knee OA.
Fifty-five male C57BL/6 mice were randomly divided into six groups: The blank control group, model control group, serum-containing hUC-MSCs (S-hUCMSC) group, N-hUCMSC group and hyaluronic acid (HA) group. After 9 weeks of modeling, the serum levels of interleukin (IL)-1β and IL-1 were determined. Hematoxylin-eosin staining was used to observe the cartilage tissue, and the Mankin score was determined. Immunohistochemistry and western blotting were used to determine the expression of collagen type II, matrix metalloproteinase (MMP)-1 and MMP-13.
The Mankin score and serum IL-1 and IL-1β and cartilage tissue MMP-1 and MMP-13 expression were significantly greater in the experimental group than in the blank control group ( < 0.05). Collagen II expression in the experimental group was significantly lower than that in the blank control group ( < 0.05). The Mankin score and serum IL-1 and IL-1β and cartilage tissue MMP-1 and MMP-13 levels the experimental group were lower than those in the model control group ( < 0.05). Collagen II expression in the experimental group was significantly greater than that in the model control group ( < 0.05).
N-hUCMSC treatment significantly alleviate the pathological damage caused by OA. The treatment effects of the S- hUCMSC group and HA group were similar.
我们研究了关节腔内注射人脐带间充质干细胞（hUC-MSCs）治疗膝关节骨关节炎（OA）进展的疗效。尽管已经发表了许多关于hUC-MSCs的实验研究，但这些研究主要使用含胎牛血清的hUC-MSCs培养物；无血清培养通常避免了含血清培养的缺点，不受伦理限制，具有广泛的临床应用前景，并为临床试验提供了基础或动物实验依据。
研究无血清hUC-MSCs（N-hUCMSCs）在小鼠膝关节OA模型中的治疗效果。
55只雄性C57BL/6小鼠随机分为6组：空白对照组、模型对照组、含血清hUC-MSCs（S-hUCMSC）组、N-hUCMSC组和透明质酸（HA）组。建模9周后，测定血清白细胞介素（IL）-1β和IL-1水平。采用苏木精-伊红染色观察软骨组织，并确定Mankin评分。采用免疫组织化学和蛋白质印迹法测定Ⅱ型胶原、基质金属蛋白酶（MMP）-1和MMP-13的表达。
实验组的Mankin评分、血清IL-1和IL-1β以及软骨组织MMP-1和MMP-13表达均显著高于空白对照组（P<0.05）。实验组Ⅱ型胶原表达显著低于空白对照组（P<0.05）。实验组的Mankin评分、血清IL-1和IL-1β以及软骨组织MMP-1和MMP-13水平低于模型对照组（P<0.05）。实验组Ⅱ型胶原表达显著高于模型对照组（P<0.05）。
N-hUCMSC治疗可显著减轻OA引起的病理损伤。S-hUCMSC组和HA组的治疗效果相似。
