Wang Dan, Wang Sinan, Sun Wenjun, Chen Tianpeng, Liang Caice, Yang Pengpeng, Liu Qingguo, Zhao Chunguang, Chen Yong
National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
Sci Rep. 2024 Dec 2;14(1):29925. doi: 10.1038/s41598-024-81490-z.
β-cyclodextrin (β-CD) is an important cyclic oligosaccharide, which is widely applicated in foods, environmental protection, and cosmetics, primarily prepared from enzymatic synthesis in traditional industry. However, several challenges persist, including cumbersome processes and difficulties in achieving continuous fermentation and catalysis. This research introduced a biofilm-based immobilized fermentation, integrating with enzyme catalysis system of surface display in Bacillus subtilis. The bslA gene was selected to construct the surface display system due to its ability to promote biofilm formation and serve as an anchorin. Compared to free cell catalysis, the biofilm-based immobilized catalysis expanded the temperature range to 40-70 and the pH range to 5-7.5. During the continuous catalysis process, by the 13th batch, the relative activity remained around 52%, and the conversion rate exceeded 36%, similar to the single-batch free cell catalysis. These findings provide valuable insights and effective strategies for the industrial production of β-CD and other biochemicals through continuous catalysis.
β-环糊精（β-CD）是一种重要的环状寡糖，广泛应用于食品、环境保护和化妆品领域，在传统工业中主要通过酶促合成制备。然而，仍然存在一些挑战，包括工艺繁琐以及实现连续发酵和催化的困难。本研究引入了基于生物膜的固定化发酵，并与枯草芽孢杆菌表面展示的酶催化系统相结合。选择bslA基因构建表面展示系统，因为它具有促进生物膜形成并作为锚定蛋白的能力。与游离细胞催化相比，基于生物膜的固定化催化将温度范围扩大到40-70，pH范围扩大到5-7.5。在连续催化过程中，到第13批时，相对活性保持在52%左右，转化率超过36%，与单批游离细胞催化相似。这些发现为通过连续催化实现β-CD和其他生物化学品的工业化生产提供了有价值的见解和有效策略。
