Zhang Yunpeng, Tang Xiaoming, Dai Jian, Li Yao, Ma Jian
Department of Orthopedic, The Affiliated Huaian No.1 people's Hospital of Nanjing Medical University, Huaian, 223300, Jiangsu, China.
Sci Rep. 2024 Dec 2;14(1):29981. doi: 10.1038/s41598-024-81844-7.
Spinal cord injury (SCI) is a profound affliction of the central nervous system that often remains inadequately addressed. Prior research has indicated that endoplasmic reticulum stress (ERS), associated with apoptotic signaling, plays a part in subsequent injuries post-SCI. However, the exact mechanisms are still unclear. ERS-related genes and SCI-associated datasets were sourced from the Genecard and GEO databases. We identified 68 ERSDEGs and pinpointed 6 marker genes vital for SCI diagnosis (CYBB, PRDX6, PTGS1, GCH1, TLR2 and PIK3CG) which were all upregulated in SCI based on bioinformatics and qRT-PCR. The nomogram exhibited that these genes could effectively predict the occurrence of SCI. Functional analysis revealed the potential roles of these genes was closely related to neuron cells and immune response. Immune infiltration research underscored the substantial roles of macrophage and CD56 dim NK cells in SCI. The ceRNA network analysis further revealed the complex interplay among marker genes, lncRNAs and miRNAs in SCI. We screened six marker genes with great diagnostic value, and found that these genes may affect the occurrence of SCI by affecting the immune response and recovery of neurons.
脊髓损伤（SCI）是中枢神经系统的一种严重疾病，目前往往仍未得到充分治疗。先前的研究表明，与凋亡信号相关的内质网应激（ERS）在脊髓损伤后的后续损伤中起作用。然而，确切机制仍不清楚。内质网应激相关基因和脊髓损伤相关数据集来自Genecard和GEO数据库。我们鉴定出68个内质网应激差异表达基因（ERSDEGs），并确定了6个对脊髓损伤诊断至关重要的标记基因（CYBB、PRDX6、PTGS1、GCH1、TLR2和PIK3CG），基于生物信息学和qRT-PCR分析，这些基因在脊髓损伤中均上调。列线图显示这些基因可以有效预测脊髓损伤的发生。功能分析表明这些基因的潜在作用与神经细胞和免疫反应密切相关。免疫浸润研究强调了巨噬细胞和CD56dim自然杀伤细胞在脊髓损伤中的重要作用。ceRNA网络分析进一步揭示了脊髓损伤中标记基因、长链非编码RNA（lncRNAs）和微小RNA（miRNAs）之间的复杂相互作用。我们筛选出六个具有重要诊断价值的标记基因，发现这些基因可能通过影响免疫反应和神经元恢复来影响脊髓损伤的发生。
