Diagnostic Ambiguity Caused by an Atypical e18a2 Transcript in a Chronic Myeloid Leukemia Patient.

We describe the case of a chronic myeloid leukemia (CML) patient with a rare atypical e18a2 :: transcript. The generation of this transcript was explained by a detailed molecular analysis, including the identification of both chromosomal breakpoints (:: on der(22) and :: on der(9)) at the genomic level. The use of a cryptic splice site in intron 1 of led to the generation of an in-frame :: fusion transcript. The diagnostic difficulties caused by this atypical variant and its implications for diagnostic routine are discussed.