Nakahashi Naoya, Emori Makoto, Takada Kohichi, Murahashi Yasutaka, Shimizu Junya, Murase Kazuyuki, Tsukahara Tomohide, Sugita Shintaro, Takasawa Akira, Iba Kousuke, Teramoto Atsushi, Osanai Makoto
Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, West 16, South 1, Chuo-ku, Sapporo, 060-8543, Japan.
Departments of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Hum Cell. 2024 Dec 3;38(1):25. doi: 10.1007/s13577-024-01157-9.
Myxofibrosarcoma (MFS) is one of the most common soft-tissue sarcomas in elderly patients. Owing to the limited efficacy of chemotherapy and radiotherapy, complete resection is the only available curative treatment. Therefore, developing novel therapies for MFS is important to improve clinical outcomes. Herein, a novel MFS cell line, namely SMU-MFS, was established to better understand the biologic characteristics of MFS and develop new therapies. A tissue sample from the surgically resected tumor tissue of a 56-year-old patient with a tumor was subjected to primary culture. The cell line was established and authenticated by assessing the short tandem repeats of DNA microsatellites. The monolayer cultures of SMU-MFS cells exhibited constant growth, spheroid formation, and invasive capacity. Furthermore, the cells exhibited low chemosensitivity to doxorubicin, eribulin, and pazopanib, which are used to inhibit metastatic progression. In addition, of the four mice inoculated with SMU-MFS cells, tumors developed in two mice after 8 weeks. Altogether, the findings of this study suggest that the SMU-MFS cell line can be a useful tool for investigating MFS development and evaluating novel therapeutic agents.
黏液纤维肉瘤（MFS）是老年患者中最常见的软组织肉瘤之一。由于化疗和放疗的疗效有限，完整切除是唯一可行的治愈性治疗方法。因此，开发针对MFS的新疗法对于改善临床结果至关重要。在此，建立了一种新的MFS细胞系，即SMU-MFS，以更好地了解MFS的生物学特性并开发新疗法。对一名56岁肿瘤患者手术切除的肿瘤组织样本进行原代培养。通过评估DNA微卫星的短串联重复序列来建立和鉴定该细胞系。SMU-MFS细胞的单层培养表现出持续生长、球体形成和侵袭能力。此外，这些细胞对用于抑制转移进展的阿霉素、艾日布林和帕唑帕尼表现出低化学敏感性。另外，在接种SMU-MFS细胞的四只小鼠中，有两只小鼠在8周后长出了肿瘤。总之，本研究结果表明，SMU-MFS细胞系可成为研究MFS发展和评估新型治疗药物的有用工具。
