Doello Sofía, Sauerwein Jakob, von Manteuffel Nathan, Burkhardt Markus, Neumann Niels, Appel Jens, Rapp Johanna, Just Pauline, Link Hannes, Gutekunst Kirstin, Forchhammer Karl
University of Tübingen, Microbiology and Organismic Interactions, Interfaculty Institute for Microbiology and Infection Medicine, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
University of Tübingen, Microbiology and Organismic Interactions, Interfaculty Institute for Microbiology and Infection Medicine, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
Curr Biol. 2025 Jan 6;35(1):77-86.e4. doi: 10.1016/j.cub.2024.11.011. Epub 2024 Dec 2.
Transitioning into and out of dormancy is a crucial survival strategy for many organisms. In unicellular cyanobacteria, surviving nitrogen-starved conditions involves tuning down their metabolism and reactivating it once nitrogen becomes available. Glucose-6-phosphate dehydrogenase (G6PDH), the enzyme that catalyzes the first step of the oxidative pentose phosphate (OPP) pathway, plays a key role in this process. G6PDH is produced at the onset of nitrogen starvation but remains inactive in dormant cells, only to be rapidly reactivated when nitrogen is restored. In this study, we investigated the mechanisms underlying this enzymatic regulation and found that G6PDH inactivation is primarily due to the accumulation of inhibitory metabolites. Moreover, our findings demonstrate that metabolite-level regulation is the driving force behind the resuscitation program. This study highlights the critical importance of metabolite-level regulation in ensuring rapid and precise enzymatic control, enabling microorganisms to swiftly adapt to environmental changes and undergo developmental transitions.
进入和脱离休眠状态是许多生物体至关重要的生存策略。在单细胞蓝细菌中，在氮饥饿条件下存活需要下调其新陈代谢，并在氮可获取时重新激活它。葡萄糖-6-磷酸脱氢酶（G6PDH）是催化氧化戊糖磷酸途径（OPP）第一步的酶，在这一过程中起关键作用。G6PDH在氮饥饿开始时产生，但在休眠细胞中保持无活性，只有在氮恢复时才迅速重新激活。在本研究中，我们研究了这种酶调控的潜在机制，发现G6PDH失活主要是由于抑制性代谢物的积累。此外，我们的研究结果表明，代谢物水平调控是复苏程序背后的驱动力。这项研究突出了代谢物水平调控在确保快速精确的酶控制方面的至关重要性，使微生物能够迅速适应环境变化并经历发育转变。
