Sadahira Y, Mori M, Awai M
Acta Pathol Jpn. 1986 Jan;36(1):23-33.
In vivo effects of anti-asialo GM1 (GA1) serum on hemopoiesis were studied by morphological examination of the blood, bone marrow, and spleen and by measuring the number of granulocyte-macrophage colony-forming units (CFU-GM) per femur after injecting of the anti-serum into mice intraperitoneally. The number of circulating lymphocytes markedly decreased 2 days after injection and then recovered, but circulating granulocytes gradually declined and reached a nadir at 6 days, then rapidly recovered. Granulopoietic activity in the bone marrow was reduced on day 2, but recovered profusely from day 4, followed by marrow neutrophilia on day 10. The spleen showed marked extramedullary granulopoiesis on days 6 and 10. CFU-GM per femur increased by 24 hours, reaching a maximum value 250% of normal on day 3, then returned to the normal level. CFU-GM growth was not affected by the treatment of anti-GA1 serum plus complement in vitro. These results suggest that the proliferating compartment of the myeloid series in the bone marrow was eliminated by anti-GA1 serum injection that led to a decrease in granulocytes and an increase in CFU-GM.
通过对血液、骨髓和脾脏进行形态学检查，以及在给小鼠腹腔注射抗血清后测量每根股骨中粒细胞 - 巨噬细胞集落形成单位（CFU - GM）的数量，研究了抗去唾液酸GM1（GA1）血清对造血作用的体内效应。注射后2天循环淋巴细胞数量显著减少，随后恢复，但循环粒细胞逐渐下降并在第6天达到最低点，然后迅速恢复。骨髓中的粒细胞生成活性在第2天降低，但从第4天开始大量恢复，随后在第10天出现骨髓嗜中性粒细胞增多。脾脏在第6天和第10天显示出明显的髓外粒细胞生成。每根股骨的CFU - GM在24小时时增加，在第3天达到正常水平的250%的最大值，然后恢复到正常水平。体外抗GA1血清加补体处理不影响CFU - GM的生长。这些结果表明，注射抗GA1血清可消除骨髓中髓系的增殖区室，导致粒细胞减少和CFU - GM增加。
