Gong Si-Yuan, Zhang Can-Xin, Xue Chen-Meng, Yang Xiao-Han, Xue Chao-Bin
Ministry of Agriculture and Rural Affairs Key Laboratory of Lower Yellow River Crop Integrated Pest Management, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China.
Arch Insect Biochem Physiol. 2024 Dec;117(4):e70007. doi: 10.1002/arch.70007.
Glucose-regulated protein 78 (Grp78), a crucial molecular chaperone in the endoplasmic reticulum, has been extensively investigated in vertebrates. However, its functional exploration in insects remains limited. This study cloned the full-length cDNA sequence of Grp78 in Plutella xylostella (L.), which is 2583 bp long. The open reading frame (ORF) is 2004 bp in length and encodes a total of 667 amino acids, including three conserved characteristic sequences of the HSP70 family. PxGrp78 is expressed in various developmental stages of P. xylostella, with the highest expression observed in third instar larvae and higher expression in female adults compared to male adults. The interference with PxGrp78 in female adults was found to significantly reduce the quantity of egg laying and the hatching rate, as well as shorten the oviposition period and down-regulate the expression of the PxVg gene. These results suggested an important role for PxGrp78 in the reproduction of P. xylostella.
葡萄糖调节蛋白78（Grp78）是内质网中的一种关键分子伴侣，已在脊椎动物中得到广泛研究。然而，其在昆虫中的功能探索仍然有限。本研究克隆了小菜蛾（Plutella xylostella (L.)）中Grp78的全长cDNA序列，长度为2583 bp。开放阅读框（ORF）长2004 bp，共编码667个氨基酸，包括HSP70家族的三个保守特征序列。PxGrp78在小菜蛾的各个发育阶段均有表达，在三龄幼虫中表达量最高，在雌成虫中的表达量高于雄成虫。研究发现，干扰雌成虫中的PxGrp78会显著降低产卵量和孵化率，缩短产卵期，并下调PxVg基因的表达。这些结果表明PxGrp78在小菜蛾繁殖中具有重要作用。
