Zucolotto Cocca Leandro H, Valverde João V P, Leite Celisnolia M, Moreno Natália S, Neto Alfredo L, Macedo Andreia G, Pratavieira Sebastião, Silva Daniel L, Rodrigues Paula C, Zucolotto Valtencir, Mendonça Cleber R, De Boni Leonardo
Photonics Group, Institute of Physics, Federal University of Goiás, Goiânia, 74690-900, GO, Brazil.
Sao Carlos Physics Institute, University of São Paulo, CP 369, 13560-970 São Carlos, SP, Brazil.
J Mater Chem B. 2025 Jan 15;13(3):1013-1023. doi: 10.1039/d4tb02291b.
The quest for novel organic fluorescent materials capable of two-photon absorption (2PA) has intensified in recent years due to their promising applications in biological imaging. Two-photon fluorescence microscopy (2PFM) offers high spatial-temporal resolution, reduced photodamage, and deeper tissue penetration compared to conventional techniques. However, the development of bright two-photon molecular markers remains a challenge, necessitating compounds with high fluorescence quantum yield and 2PA cross-section (). Strategies such as increasing π-conjugation have shown promise but are hindered by synthesis complexities and limited biocompatibility. Alternatively, incorporating electron-donating (ED) or electron-withdrawing (EW) peripheral groups in a main structure has emerged as a viable approach, leading to significant enhancements in . This study highlights the advantages and challenges of these strategies, emphasizing the importance of exploring new organic compounds and evaluating the efficacy of peripheral groups for advanced two-photon bioimaging applications.
近年来，由于其在生物成像方面的应用前景广阔，对能够进行双光子吸收（2PA）的新型有机荧光材料的探索日益激烈。与传统技术相比，双光子荧光显微镜（2PFM）具有高时空分辨率、减少光损伤以及更深的组织穿透深度。然而，开发明亮的双光子分子标记物仍然是一项挑战，需要具有高荧光量子产率和双光子吸收截面（）的化合物。增加π共轭等策略已显示出前景，但受到合成复杂性和有限生物相容性的阻碍。另外，在主结构中引入供电子（ED）或吸电子（EW）外围基团已成为一种可行的方法，导致在方面有显著提高。本研究强调了这些策略的优点和挑战，强调了探索新有机化合物以及评估外围基团对先进双光子生物成像应用效果的重要性。
