Fushuku Hiroki, Matsunaga Nobuyoshi
Department of Life and Food Sciences, School of Agriculture and Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Anim Sci J. 2024 Jan-Dec;95(1):e70016. doi: 10.1111/asj.70016.
This study was designed to investigate the degree of long-term effects by measuring cortisol and aldosterone concentrations in the growing hair including lipid which is absorbed from the blood of six female Holstein calves by enzyme-linked immunosorbent assay (ELISA). The total number of calves used was 18 (three seasons). Three comparative factors were used: region (back and chest), hair color (black and white), and season (summer, winter, and spring). The hair cortisol of the back region (white color) was higher than the other region and color (p < 0.05). The measured value in the spring season (back region) was significantly higher than that in the summer (chest region) (p < 0.05). Although there has been no report on the hair aldosterone until now, it was possible to measure the concentration in the present study. The hair aldosterone of the back region was higher than the chest region (p < 0.01), and the white hair color was higher than the black hair color (p < 0.05). The measured value in the spring season was also significantly higher than that in the summer season and the winter season (p < 0.01). This result suggested the changes are affected by the hair region factor, the hair color factor, and the changing meteorological conditions.
本研究旨在通过酶联免疫吸附测定法（ELISA）测量六头雌性荷斯坦犊牛生长毛发中的皮质醇和醛固酮浓度（包括从血液中吸收的脂质），以调查长期影响的程度。使用的犊牛总数为18头（三个季节）。采用了三个比较因素：部位（背部和胸部）、毛色（黑色和白色）和季节（夏季、冬季和春季）。背部区域（白色）的毛发皮质醇高于其他区域和毛色（p<0.05）。春季（背部区域）的测量值显著高于夏季（胸部区域）（p<0.05）。虽然目前尚无关于毛发醛固酮的报道，但在本研究中能够测量其浓度。背部区域的毛发醛固酮高于胸部区域（p<0.01），白色毛色高于黑色毛色（p<0.05）。春季的测量值也显著高于夏季和冬季（p<0.01）。该结果表明，这些变化受毛发部位因素、毛色因素和气象条件变化的影响。
