Yuan Sheng, Huang Ying, Wang Quan, Shi Yan, Song Xiao-Rui, Zhao Zhe
Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing, 210024, China; Department of Aquatic Science and Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, 225300, China.
Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing, 210024, China.
Fish Shellfish Immunol. 2025 Feb;157:110070. doi: 10.1016/j.fsi.2024.110070. Epub 2024 Dec 3.
The S100 proteins are highly conserved EF-hand calcium-binding proteins found only in vertebrates. In the current study, two S100 genes (S100A1 and S100A10) were successfully identified and characterized from hybrid grouper Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀. The deduced S100A10 protein contained two EF-hand domains, and S100A1 only possessed the N-terminal EF-hand. Phylogenetic analysis revealed that S100A1 and S100A10 from hybrid grouper were evolutionarily closely related to their counterparts in other selected vertebrates. Quantitative real-time PCR results revealed that the transcripts of S100A1 and S100A10 mRNA were ubiquitously distributed in all the examined tissues. After Vibrio alginolyticus infection, the expression of S100A1 and S100A10 in the spleen increased significantly. Moreover, overexpression of S100A1 and S100A10 could not only regulate the expression of interleukin 8 (IL-8), IL-10, and IL-16 in the head kidney, liver, and spleen, change the activities of acid phosphatase, catalase, lysozyme, and superoxide dismutase in serum, but also reduce the promoter activities of interferon 3 and nuclear factor kappa-B in vitro. Taken together, this study indicated that S100A1 and S100A10 participate in the immune response of hybrid grouper against bacterial infection.
S100蛋白是仅在脊椎动物中发现的高度保守的EF手型钙结合蛋白。在本研究中，成功地从鞍带石斑鱼♂×褐点石斑鱼♀杂交石斑鱼中鉴定并表征了两个S100基因（S100A1和S100A10）。推导的S100A10蛋白包含两个EF手型结构域，而S100A1仅具有N端EF手型结构域。系统发育分析表明，杂交石斑鱼的S100A1和S100A10在进化上与其在其他选定脊椎动物中的对应物密切相关。实时定量PCR结果显示，S100A1和S100A10 mRNA的转录本在所有检测组织中均有广泛分布。溶藻弧菌感染后，脾脏中S100A1和S100A10的表达显著增加。此外，S100A1和S100A10的过表达不仅可以调节头肾、肝脏和脾脏中白细胞介素8（IL-8）、IL-10和IL-16的表达，改变血清中酸性磷酸酶、过氧化氢酶、溶菌酶和超氧化物歧化酶的活性，还可以在体外降低干扰素3和核因子κB的启动子活性。综上所述，本研究表明S100A1和S100A10参与了杂交石斑鱼对细菌感染的免疫反应。
