Jeong Ji-Won, Park Dong-Joo, Kim Se-Chang, Kang Hyun Wook, Lee Bonggi, Kim Hyun-Woo, Kim Young-Mog, Linh Nguyen Vu, Jung Won-Kyo
Research Center for Marine-Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea.
Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence and New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea; Marine integrated Biomedical Technology center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea.
Int J Biol Macromol. 2025 Jan;286:138254. doi: 10.1016/j.ijbiomac.2024.138254. Epub 2024 Dec 3.
Various wound dressings are under development to accelerate wound healing, and hydrogels in particular have the potential to create ideal conditions for wound healing. In this study, we investigated a novel hydrogel based on gelatin/oxidized carboxymethyl cellulose loaded with fucoidan derived from Ecklonia cava (ECF) for wound treatment. The mechanical stability and self-healing ability of the hydrogel were optimized, and its cytocompatibility was demonstrated against RAW 264.7 macrophages and human dermal fibroblasts (HDF). Furthermore, sustained drug release from the fabricated hydrogels depending on ECF concentration exhibited radical scavenging ability, induction of collagen production, promotion of cell migration, reduction of nitric oxide levels, and cytoprotective effects against oxidative stress in the ROS microenvironment, attributing these effects to the wound healing potential of bioactive ECF. In an in vivo experiments, GOC/F5 hydrogel was shown to significantly reduce wound area in a full-thickness ICR mouse model, and promoted re-epithelialization and collagen deposition to rapidly repair wounds. These results suggest that GOC/F5 hydrogels could be potentially used as an ideal dressing for wound healing.
目前正在研发各种伤口敷料以加速伤口愈合，尤其是水凝胶有潜力为伤口愈合创造理想条件。在本研究中，我们研究了一种基于明胶/氧化羧甲基纤维素并负载有源自海蕴的岩藻依聚糖（ECF）的新型水凝胶用于伤口治疗。优化了水凝胶的机械稳定性和自愈能力，并证明了其对RAW 264.7巨噬细胞和人皮肤成纤维细胞（HDF）的细胞相容性。此外，根据ECF浓度，制备的水凝胶的持续药物释放表现出自由基清除能力、诱导胶原蛋白生成、促进细胞迁移、降低一氧化氮水平以及在ROS微环境中对氧化应激的细胞保护作用，将这些作用归因于生物活性ECF的伤口愈合潜力。在体内实验中，GOC/F5水凝胶在全层ICR小鼠模型中显示出显著减小伤口面积的作用，并促进上皮再形成和胶原蛋白沉积以快速修复伤口。这些结果表明，GOC/F5水凝胶有可能用作伤口愈合的理想敷料。
