Chen Wenqin, Li Zheqian, Zheng Jie, Wang Danwei, Gao Shuo, Zhou Wanqing, Shen Han, Zhang Yan, Cao Xiaoli
Department of Clinical Laboratory, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu Province, PR China.
Department of Clinical Laboratory, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, PR China.
J Glob Antimicrob Resist. 2025 Jan;40:81-89. doi: 10.1016/j.jgar.2024.11.013. Epub 2024 Dec 2.
Serratia marcescens outbreaks present significant challenges in clinical treatment, necessitating a deeper understanding of its epidemiological and genomic traits.
To analyse the epidemiological and genomic characteristics of S. marcescens at a global scale.
High-quality genomes of S. marcescens were retrieved from NCBI and annotated using Prodigal. Antibiotic resistance genes (ARGs) were identified via Blastn, sequence types (STs) were determined with a proprietary tool, and phylogenetic analysis was conducted to explore evolutionary relationships.
The study analysed genomes from 33 countries, with major contributions from the USA (27.8%), UK (15.3%), Italy (14.7%), and Japan (10.7%). Human clinical samples accounted for 73.5% of the isolates, primarily from blood (44.8%) and sputum (19.3%). Eleven ARGs were identified, with sde being the most prevalent. Carbapenemase genes included blaSME, blaKPC, and blaNDM-1, though co-occurrence in individual strains was absent. Novel ARGs, including armA, rmtC, and fosA7.2, were reported. Among 855 genomes with identified STs, ST366, ST367, ST365, and ST423 were most common. Phylogenetic analysis highlighted significant genetic diversity and distinct evolutionary lineages.
Temporal analysis showed a genome peak in 2019, underscoring the global prevalence and adaptability of S. marcescens. The distribution of ARGs across diverse STs emphasizes horizontal gene transfer as a key driver of resistance. Judicious antibiotic use is essential to mitigate further resistance.
粘质沙雷氏菌爆发给临床治疗带来重大挑战，因此有必要更深入地了解其流行病学和基因组特征。
在全球范围内分析粘质沙雷氏菌的流行病学和基因组特征。
从NCBI检索高质量的粘质沙雷氏菌基因组，并使用Prodigal进行注释。通过Blastn鉴定抗生素抗性基因（ARG），使用专有工具确定序列类型（ST），并进行系统发育分析以探索进化关系。
该研究分析了来自33个国家的基因组，其中美国（27.8%）、英国（15.3%）、意大利（14.7%）和日本（10.7%）贡献较大。人类临床样本占分离株的73.5%，主要来自血液（44.8%）和痰液（19.3%）。鉴定出11个ARG，其中sde最为普遍。碳青霉烯酶基因包括blaSME、blaKPC和blaNDM-1，不过在单个菌株中未同时出现。报告了新的ARG，包括armA、rmtC和fosA7.2。在855个已鉴定ST的基因组中，ST366、ST367、ST365和ST423最为常见。系统发育分析突出了显著的遗传多样性和不同的进化谱系。
时间分析显示2019年基因组出现峰值，凸显了粘质沙雷氏菌的全球流行和适应性。ARG在不同ST中的分布强调水平基因转移是耐药性的关键驱动因素。明智地使用抗生素对于减轻进一步的耐药性至关重要。
