Wang Qin, Salman Sayyed, Luo Ye, Tong XiaoMei
Center of Reproductive Medicine, Sir Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
J Glob Antimicrob Resist. 2025 Jan;40:37-41. doi: 10.1016/j.jgar.2024.11.006. Epub 2024 Dec 2.
The Enterobacter cloacae complex, known for causing infections in hospitalised patients, displays resistance to β-lactam antibiotics due to AmpC β-lactamase expression. This study emphasises the genome sequence of Enterobacter roggenkampii, which coexists with MIR-1 and NDM-1 genes, isolated from the stool of a hospitalised patient in China.
A faecal sample was diluted in Luria-Bertani broth and incubated overnight. Cultures were then spread on MacConkey agar containing meropenem and incubated for 18-24 h to select carbapenem-resistant Enterobacteriaceae. Individual colonies were isolated, and bacterial species were identified using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and 16S rRNA gene sequencing. Genomic DNA was extracted and sequenced via Illumina and Oxford Nanopore sequencing.
Genomic analysis of E. roggenkampii L3897 revealed a 4 897 636 bp genome with 55.9% GC content and confirmed its classification as E. roggenkampii through average nucleotide identity-based analysis. A new sequence type distinct from ST2392, ST3014, with a unique rplB genotype was identified. Additionally, the genome harbours three antibiotic resistance genes and a newly discovered plasmid, pL3897_NDM, highlighting the need for surveillance of drug-resistant pathogens.
The discovery of a new sequence type and the presence of an antibiotic resistance gene in E. roggenkampii L3897 underscores the need for ongoing genomic surveillance to effectively manage multidrug-resistant pathogens.
阴沟肠杆菌复合体因可导致住院患者感染而闻名，由于其表达AmpC β-内酰胺酶，对β-内酰胺类抗生素具有耐药性。本研究着重介绍了从中国一名住院患者粪便中分离出的与MIR-1和NDM-1基因共存的罗根坎普肠杆菌的基因组序列。
将一份粪便样本在Luria-Bertani肉汤中稀释并孵育过夜。然后将培养物接种在含美罗培南的麦康凯琼脂上，孵育18 - 24小时以筛选耐碳青霉烯类肠杆菌科细菌。分离出单个菌落，使用基质辅助激光解吸/电离飞行时间质谱和16S rRNA基因测序鉴定细菌种类。提取基因组DNA并通过Illumina和牛津纳米孔测序进行测序。
对罗根坎普肠杆菌L3897的基因组分析显示其基因组大小为4 897 636 bp，GC含量为55.9%，通过基于平均核苷酸同一性的分析确认其为罗根坎普肠杆菌。鉴定出一种不同于ST2392、ST3014的新序列类型ST3014，具有独特的rplB基因型。此外，该基因组含有三个抗生素抗性基因和一个新发现的质粒pL3897_NDM，凸显了对耐药病原体进行监测的必要性。
罗根坎普肠杆菌L3897中发现新序列类型以及抗生素抗性基因的存在，强调了持续进行基因组监测以有效管理多重耐药病原体的必要性。
