Hong Minhua, Gui Yingyue, Xu Jiayao, Zhao Xianglong, Jiang Chunyang, Zhao Jian, Xin Xiujuan, Liu Dan, Tang Xiaolin, Tang Rong, An Faliang
Shanghai Peptide Biotechnology Co. Ltd, 1777 Hualong Road, Shanghai, 201705, China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Biochem Biophys Res Commun. 2025 Jan;742:151060. doi: 10.1016/j.bbrc.2024.151060. Epub 2024 Nov 28.
Copper peptide, a low molecular weight peptide composed of glycyl-L-histidyl-l-lysine-copper, possesses anti-aging, anti-inflammatory, and wound healing properties. In this study, we investigated the effects of a combination agent CP-AcT, composed of palmitoyl copper peptide (pal-GHK-Cu) and acetyl tyrosine (N-Acetyl-l-tyrosine), on melanin production in the human malignant melanoma cell line A375 and the mouse melanoma cell line B16. Firstly, the cytotoxicity of CP-AcT at various concentrations (0-8 μg/mL) on HaCat, HFF, A375, and B16 cells was evaluated. Subsequently, the effects of the CP-AcT on tyrosinase activity both extracellular and intracellularly, as well as on melanin production in two melanoma cell lines, were evaluated under conditions that did not compromise cell viability. Additionally, quantitative gene plex (QGP) combined with branched DNA (bDNA) technology was used to analyze the effects of CP-AcT on the expression of melanin-related genes in A375 cells, with a focus on five specific genes. Finally, the effects of the CP-AcT on the expression of three proteins involved in the biosynthesis pathway of melanin: tyrosinase (TYR), dopachrome tautomerase (DCT), and endothelin 3 (EDN3) were analyzed. The results indicate that the complex CP-AcT effectively promotes melanin production in both types of melanoma cells.
铜肽是一种由甘氨酰-L-组氨酰-L-赖氨酸-铜组成的低分子量肽，具有抗衰老、抗炎和伤口愈合特性。在本研究中，我们研究了由棕榈酰铜肽（pal-GHK-Cu）和乙酰酪氨酸（N-乙酰-L-酪氨酸）组成的复合剂CP-AcT对人恶性黑色素瘤细胞系A375和小鼠黑色素瘤细胞系B16中黑色素生成的影响。首先，评估了不同浓度（0-8μg/mL）的CP-AcT对HaCat、HFF、A375和B16细胞的细胞毒性。随后，在不影响细胞活力的条件下，评估了CP-AcT对细胞外和细胞内酪氨酸酶活性以及对两种黑色素瘤细胞系中黑色素生成的影响。此外，使用定量基因复合物（QGP）结合分支DNA（bDNA）技术分析CP-AcT对A375细胞中黑色素相关基因表达的影响，重点关注五个特定基因。最后，分析了CP-AcT对黑色素生物合成途径中涉及的三种蛋白质：酪氨酸酶（TYR）、多巴色素互变异构酶（DCT）和内皮素3（EDN3）表达的影响。结果表明，复合剂CP-AcT有效促进了两种类型黑色素瘤细胞中的黑色素生成。
