Teske Christian, Liedel Katja, Hirle Alexander, Baenke Franziska, Stange Daniel E, Weitz Jürgen, Preusse Grit, Steiner Gerald
Department of Visceral, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
National Center for Tumor Diseases (NCT/UCC), German Cancer Research Center (DKFZ), Faculty of Medicine and University Hospital Carl Gustav Carus, Helmholtz-Zentrum Dresden - Rossendorf (HZDR), Dresden, Germany.
Cancer Med. 2024 Dec;13(23):e70457. doi: 10.1002/cam4.70457.
Pancreatic ductal adenocarcinoma (PDAC) continues to pose profound challenges within the field of oncology due to its notorious resistance to existing therapies and constant high mortality rates. The recent emergence of three-dimensional patient-derived organoid (PDO) models marks a significant advancement, opening new avenues for exploring cancer biology and assessing therapeutic approaches.
The aim of this study focuses on the innovative use of Fourier-transform infrared (FT-IR) spectroscopy to analyze PDAC organoids, thus illuminating their biochemical intricacies.
In this study, PDAC organoids, cultivated from specimens sourced from cancer patients, were subjected to FT-IR spectroscopic imaging. By examining the spectral data within the critical fingerprint region (950-1800 cm), and employing principal component analysis (PCA), biochemical disparities were detected and analyzed.
The results revealed distinct spectral profiles corresponding to different sample preparation techniques, which in turn highlighted variations in protein content and structure. PCA revealed a high homogeneity within classes and minimal passage number influence on spectral profiles, with variations in lipid content and protein profiles. Significantly, the biochemical fingerprint of these PDOs closely mirrored that of the original human tissue samples.
This investigation underscores the efficacy of molecular spectroscopy as a non-invasive method for profound characterization of PDAC organoids, enhancing our comprehension of tumor biochemistry. The capacity for swift and precise biochemical profiling of PDOs via molecular spectroscopy heralds a promising future for this technique in the realms of cancer diagnostics and personalized medicine.
胰腺导管腺癌（PDAC）因其对现有治疗方法的显著抗性和持续的高死亡率，继续在肿瘤学领域构成严峻挑战。三维患者来源类器官（PDO）模型的近期出现标志着一项重大进展，为探索癌症生物学和评估治疗方法开辟了新途径。
本研究的目的集中于傅里叶变换红外（FT-IR）光谱的创新应用，以分析PDAC类器官，从而阐明其生化复杂性。
在本研究中，对从癌症患者标本中培养的PDAC类器官进行FT-IR光谱成像。通过检查关键指纹区域（950 - 1800 cm）内的光谱数据，并采用主成分分析（PCA），检测并分析生化差异。
结果显示，对应于不同样品制备技术的光谱特征不同，这反过来突出了蛋白质含量和结构的变化。PCA显示类别内高度均匀，传代次数对光谱特征的影响最小，脂质含量和蛋白质谱存在差异。重要的是，这些PDO的生化指纹与原始人体组织样本的生化指纹非常相似。
本研究强调了分子光谱作为一种非侵入性方法对PDAC类器官进行深度表征的有效性，增强了我们对肿瘤生物化学的理解。通过分子光谱对PDO进行快速准确的生化分析的能力预示着该技术在癌症诊断和个性化医疗领域的光明前景。
