通过超高灵敏度电视摄像系统对二维薄层色谱板上的³H和¹⁴C进行定量荧光检测。

Quantitative fluorographic detection of 3H and 14C on two-dimensional thin-layer chromatographic sheets by an ultra-high-sensitivity TV camera system.

作者信息

Miwa M, Matsumoto M, Tezuka M, Okada S, Ohsuka S, Fujiwake H

出版信息

Anal Biochem. 1986 Feb 1;152(2):391-5. doi: 10.1016/0003-2697(86)90425-2.

Abstract

A method for quantitative detection of 3H and 14C on thin layers is described. After impregnation of the TLC sheet with 50% 2,5-diphenyloxazole-tetrahydrofuran, quantitative imaging of the distribution of weak beta-ray-emitting isotopes on the chromatogram was carried out at room temperature by using a TV camera system, which consisted of a two-stage microchannel plate image intensifier, a low-lag vidicon, and an image processor. The method is applicable for 14C- and 3H-labeled samples on TLC sheets (10 X 10 cm) emitting more than 0.17 and 7.5 Bq/mm2, respectively. The method is rapid and has a dynamic range far greater than that of film.

摘要

描述了一种在薄层上定量检测³H和¹⁴C的方法。用50% 2,5 - 二苯基恶唑 - 四氢呋喃浸渍薄层层析板后，在室温下使用由两级微通道板图像增强器、低滞后光导摄像管和图像处理器组成的电视摄像系统对色谱图上发射弱β射线的同位素分布进行定量成像。该方法适用于分别发射超过0.17和7.5 Bq/mm²的¹⁴C和³H标记的薄层层析板（10×10 cm）上的样品。该方法快速，动态范围远大于胶片。