细胞对3-羟基苯并[a]芘的葡萄糖醛酸化作用：培养基中的直接荧光测定法。
3-Hydroxybenzo[a]pyrene glucuronidation by cells: a direct fluorometric assay in culture medium.
作者信息
Boiret M, Marty A, Deumié M
出版信息
Anal Biochem. 1986 Feb 15;153(1):132-8. doi: 10.1016/0003-2697(86)90071-0.
PMID:3963376
Abstract
A direct fluorometric assay for measuring the glucuronidation by cultured cells using 3-hydroxybenzo[a]pyrene (less than 1 microM) as substrate is described. The method is based on the different characteristics in the fluorescence spectra of 3-hydroxybenzo[a]pyrene and benzo[a]pyrene-3-glucuronide. The analytical procedure operates directly on the culture medium without extraction steps. The sensitivity of detection is 1 nmol glucuronide/1 mumol substrate.
摘要
描述了一种直接荧光测定法，用于测量培养细胞以3-羟基苯并[a]芘（小于1微摩尔）为底物的葡萄糖醛酸化作用。该方法基于3-羟基苯并[a]芘和苯并[a]芘-3-葡萄糖醛酸苷荧光光谱的不同特征。分析过程直接在培养基上进行，无需萃取步骤。检测灵敏度为1纳摩尔葡萄糖醛酸苷/1微摩尔底物。
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