Joppich M, Joppich-Kuhn R, Sentissi A, Giese R W
Anal Biochem. 1986 Feb 15;153(1):159-65. doi: 10.1016/0003-2697(86)90075-8.
Reaction of thyroxine with ethanol and pivalic anhydride in the presence of 4-dimethylaminopyridine quantitatively forms N,O-dipivalyl thyroxine ethyl ester. Other iodothyronines react similarly and the procedure is moisture insensitive. Apparently this reaction is successful, in contrast to similar procedures reported for the derivatization of alpha-amino acids, because it overcomes the problem in other procedures of irreversible side reactions arising from an oxazolone intermediate.
在4-二甲氨基吡啶存在下，甲状腺素与乙醇和新戊酸酐反应定量生成N,O-二新戊酰甲状腺素乙酯。其他碘甲状腺原氨酸也有类似反应，且该过程对水分不敏感。显然，与报道的α-氨基酸衍生化的类似方法相比，该反应是成功的，因为它克服了其他方法中由恶唑酮中间体引起的不可逆副反应问题。
