McKinney L C, Chakraverty S, De Weer P
Anal Biochem. 1986 Feb 15;153(1):33-8. doi: 10.1016/0003-2697(86)90056-4.
The alkaloid neurotoxin veratridine is widely used by cell physiologists to increase membrane sodium permeability. The compound is only sporadically available from commercial sources, but can be purified (Kupchan et al., 1953, J. Amer. Chem. Soc. 75, 5519-5524) from veratrine, a mixture of several alkaloids. We describe here a purification procedure only slightly modified from that of Kupchan et al., and include important details not mentioned in the original paper. Ultraviolet and infrared spectra are presented. We have also determined the pKa and solubility of veratridine in 150 mM NaCl at 25 degrees C. The solubility is steeply pH dependent, ranging from 0.61 +/- 0.02 mM above pH 12 to 18.5 mM at pH 8.07. The pKa, determined from the solubility versus pH curve, was found to be 9.54 +/- 0.02.
生物碱神经毒素藜芦定被细胞生理学家广泛用于增加细胞膜对钠的通透性。该化合物仅偶尔能从商业渠道获得，但可从藜芦碱（几种生物碱的混合物）中纯化得到（库普昌等人，1953年，《美国化学会志》75卷，5519 - 5524页）。我们在此描述一种仅对库普昌等人的方法稍作修改的纯化程序，并包含原始论文中未提及的重要细节。给出了紫外和红外光谱。我们还测定了藜芦定在25℃下于150 mM氯化钠中的pKa和溶解度。溶解度强烈依赖于pH值，在pH值高于12时为0.61±0.02 mM，在pH 8.07时为18.5 mM。由溶解度与pH值曲线确定的pKa为9.54±0.02。
