Mohamed Nashwa Farouk, Ali Behairy Ola Galal, Sadek El Defrawy Manal, Elsheraki Samar Ahmed Mohamed, Khashaba Rana Atef
Pediatrics Department, Faculty of Medicine, Benha University, Benha, Egypt.
Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt.
Scand J Clin Lab Invest. 2024 Nov-Dec;84(7-8):515-520. doi: 10.1080/00365513.2024.2437612. Epub 2024 Dec 5.
The aim of this study was to evaluate the role of serum human epididymis protein 4 (HE4) as a non-invasive biomarker for the diagnosis of liver fibrosis in children with chronic liver diseases (CLD). This case-control study was conducted at Benha University Hospital, Egypt, involving 60 children with CLD and 60 healthy children as a control group. HE4 levels were measured by ELISA and compared with liver biopsy results. The CLD group had significant higher HE4 (median: 110.7, IQR: 96.7-120.4 pmol/L) compared to control group (median 42.07, IQR: 41.67-43.05 pmol/L), < .001. HE4 levels increased significantly with the degree of fibrosis and histological activity index. At a cutoff point >48.3 pmol/L, HE4 diagnosed cases with mild fibrosis with a sensitivity of 95% and specificity of 91.3%. At a cutoff point >144.3 pmol/L, HE4 diagnosed cases with severe fibrosis with a sensitivity of 98% and specificity of 93.1%. Serum HE4 is a potential non-invasive marker for detecting liver fibrosis and its severity in children with CLD.
本研究旨在评估血清人附睾蛋白4（HE4）作为慢性肝病（CLD）患儿肝纤维化诊断的非侵入性生物标志物的作用。本病例对照研究在埃及本哈大学医院进行，纳入60例CLD患儿和60例健康儿童作为对照组。采用酶联免疫吸附测定法（ELISA）检测HE4水平，并与肝活检结果进行比较。与对照组（中位数42.07，四分位间距：41.67 - 43.05 pmol/L）相比，CLD组的HE4水平显著更高（中位数：110.7，四分位间距：96.7 - 120.4 pmol/L），P < .001。HE4水平随纤维化程度和组织学活动指数显著升高。在截断点>48.3 pmol/L时，HE4诊断轻度纤维化病例的敏感性为95%，特异性为91.3%。在截断点>144.3 pmol/L时，HE4诊断重度纤维化病例的敏感性为98%，特异性为93.1%。血清HE4是检测CLD患儿肝纤维化及其严重程度的潜在非侵入性标志物。
