沙门氏菌噬菌体P22对外源DNA的体外包装

In vitro packaging of exogenous DNA by Salmonella phage P22.

作者信息

Strobel E, Schmieger H

出版信息

J Gen Virol. 1979 Nov;45(2):291-9. doi: 10.1099/0022-1317-45-2-291.

Abstract

We describe in vitro conditions for packaging of exogenous DNA of Salmonella phage P22 which has terminally redundant, circularly permuted DNA. The method is a modification of the Kaiser-Masuda procedure. The most important aspect is to prepare all components (proheads, enzymes and concatemeric DNA) in end- cells. The influence of several factors such as DNA- and Mg2+ concentration and kinetics has been investigated.

摘要

我们描述了用于包装具有末端冗余、环状排列DNA的沙门氏菌噬菌体P22外源DNA的体外条件。该方法是对Kaiser-Masuda程序的改进。最重要的方面是在无细胞体系中制备所有成分（前噬菌体头部、酶和串联DNA）。已经研究了几个因素的影响，如DNA和Mg2+浓度以及动力学。