Wang Deqing, Su Dianbin, Xu Huihui, Chen Xiaofeng, Lv Weiqiao, Wang Yong, Sun Xia, Guo Yemin, Ding Yukuan
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Int J Biol Macromol. 2025 Jan;285:138306. doi: 10.1016/j.ijbiomac.2024.138306. Epub 2024 Dec 3.
Drying techniques have been widely used in food macromolecule modification, sterilization, and other fields. However, comparisons about the effects of different heat treatments on the structural and functional properties of proteins have yet to be elucidated. In this study, we compared the effects of boiling water, microwave, hot air, and infrared pretreatment on the structural and functional properties of P. eryngii (Pleurotus eryngii) proteins (PEP), with the addition of low-temperature hot air drying (Fresh-H) and vacuum freeze drying (VFD) as controls. The flavor of apricot mushroom products was also analyzed. The results showed that the PEP from boiling water pretreatment combined with hot air drying (BPHD) maintained high surface hydrophobicity, turbidity, particle size, and zeta potential. Microwave pretreatment combined with hot air drying (MPHD) of PEP had higher water and oil-holding properties, crystallinity, hydrolysis, and antioxidant capacity. Meanwhile, both MPHD and infrared pretreatment combined with hot air drying (IPHD) maintained higher emulsification, foaming, and free amino acid content. There were large differences in the types and contents of volatile compounds in P. eryngii with different treatments, providing more options.
干燥技术已广泛应用于食品大分子改性、杀菌等领域。然而,不同热处理对蛋白质结构和功能特性影响的比较尚待阐明。在本研究中,我们比较了沸水、微波、热风和红外预处理对杏鲍菇(刺芹侧耳)蛋白(PEP)结构和功能特性的影响,并添加了低温热风干燥(Fresh-H)和真空冷冻干燥(VFD)作为对照。还分析了杏鲍菇产品的风味。结果表明,经沸水预处理并结合热风干燥(BPHD)得到的PEP保持了较高的表面疏水性、浊度、粒径和zeta电位。经微波预处理并结合热风干燥(MPHD)的PEP具有较高的持水和持油性能、结晶度、水解度和抗氧化能力。同时,MPHD和经红外预处理并结合热风干燥(IPHD)的PEP均保持了较高的乳化、发泡和游离氨基酸含量。不同处理的杏鲍菇中挥发性化合物的种类和含量存在较大差异,提供了更多选择。