Dewasmika W A Pamudi Maldam, Dissanayake D R Anuruddhika, Neelawala N G Dulanjalee Anuruddhika Kumari, Dissanayake B G Nilanthi, Prasada D V Pahan, Francis Veithehi Rajeevan, Octavia Sophie, Lan Ruiting
Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya Sri Lanka, Peradeniya, Sri Lanka.
Department of Microbiology, Faculty of Medicine University of Peradeniya, Peradeniya, Sri Lanka.
Zoonoses Public Health. 2025 Mar;72(2):200-206. doi: 10.1111/zph.13199. Epub 2024 Dec 5.
Extraintestinal pathogenic Escherichia coli (ExPEC) is an important human and animal pathogen. In particular, the ST131 of ExPEC is a widely distributed clone, resistant to extended-spectrum cephalosporins and fluoroquinolones. We investigated the occurrence of ST131 among ExPEC from humans and dogs in Sri Lanka and determined its antimicrobial resistance.
A total of 215 ExPEC isolates were collected from humans (n = 179) and dogs (n = 36) from two different cities in Sri Lanka. Antimicrobial resistance was tested by the disk diffusion method, and the presence of resistance-encoding genes (bla, bla and bla) and ST131 clades/subclades was tested by PCR.
The majority of the isolates were (61.8%) ST131, with 55.8% in ST131-clade C. Of the clade C isolates, 44.3%, 27.8%, 15.7% and 6.7% were in clades C2, C other, C1-non-M27 and C1-M27, respectively. Approximately 11% of the ST131 isolates were carbapenem resistant. ExPEC from dogs showed comparable resistance rates to human isolates except for resistance to amoxicillin-clavulanate and amikacin.
We observed a high occurrence of ST131 and its clade C, with more than 11% exhibiting resistance to carbapenems in Sri Lanka. Furthermore, ST131-C1-M27, with high resistance to both quinolones and extended-spectrum cephalosporins, was also present. Our results emphasise the importance of the One Health approach in the monitoring of antimicrobial-resistant E. coli in different regions of Sri Lanka to gain a better understanding of their prevalence over time, contributing to effective antimicrobial stewardship.
肠外致病性大肠杆菌（ExPEC）是一种重要的人类和动物病原体。特别是，ExPEC的ST131是一种广泛分布的克隆株，对超广谱头孢菌素和氟喹诺酮类耐药。我们调查了斯里兰卡人类和犬类ExPEC中ST131的发生情况，并确定了其抗菌药物耐药性。
从斯里兰卡两个不同城市的人类（n = 179）和犬类（n = 36）中总共收集了215株ExPEC分离株。采用纸片扩散法检测抗菌药物耐药性，通过PCR检测耐药编码基因（bla、bla和bla）的存在情况以及ST131分支/亚分支。
大多数分离株（61.8%）为ST131，其中55.8%属于ST131-C分支。在C分支分离株中，分别有44.3%、27.8%、15.7%和6.7%属于C2、其他C、C1-非M27和C1-M27分支。约11%的ST131分离株对碳青霉烯类耐药。除对阿莫西林-克拉维酸和阿米卡星耐药外，犬类ExPEC的耐药率与人类分离株相当。
我们观察到ST131及其C分支在斯里兰卡的发生率很高，超过11%对碳青霉烯类耐药。此外，对喹诺酮类和超广谱头孢菌素均具有高耐药性的ST131-C1-M27也存在。我们的结果强调了“同一健康”方法在监测斯里兰卡不同地区耐抗菌药物大肠杆菌方面的重要性，以便更好地了解其随时间的流行情况，有助于有效的抗菌药物管理。
