Sharifi Aram, Mahmoudi Peyman, Sobhani Keyvan, Ashengroph Morahem
Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Kurdistan, Iran.
Department of Biology, Faculty of Science, University of Kurdistan, Sanandaj, Kurdistan, Iran.
Microbiol Immunol. 2025 Feb;69(2):104-113. doi: 10.1111/1348-0421.13189. Epub 2024 Dec 5.
Staphylococcus aureus is a versatile pathogen capable of causing a wide range of infections, from minor skin infections to life-threatening invasive diseases. The pathogenicity of S. aureus is attributed to its ability to produce various virulence factors, including adhesion and biofilm-related proteins. Understanding the prevalence and distribution of these genes among S. aureus isolates from different sources is crucial for devising effective strategies to combat biofilm-associated contamination. In this study, we conducted a comprehensive network meta-analysis to assess the prevalence of adhesion and biofilm-related genes in S. aureus isolates and investigate the impact of the isolate source on their occurrence. A systematic search of multiple databases was performed, and a total of 53 relevant studies were included. The prevalence of adhesion and biofilm-related genes in S. aureus isolates was determined, with the highest prevalence observed for clfB (p-estimate = 85.4, 95% confidence interval [CI] 78-90.6), followed by eno (p-estimate = 81.1, 95% CI 61.7-91.9), and icaD (p-estimate = 77, 95% CI 68.6-83.6). Conversely, bap and bbp genes exhibited the lowest prevalence rates (p-estimate = 6.7 and 18.7, respectively). The network meta-analysis allowed us to examine the pairwise co-study of adhesion and biofilm-related genes in S. aureus isolates. The most frequently co-studied gene pairs were icaA-icaD (30 times) and fnbA-fnbB (25 times). Subgroup analysis showed that the occurrence of icaC and icaB genes was significantly lower in animal isolates compared to human and food isolates (p < 0.05). It is worth noting that there was limited data available for the analysis of sasG, bbp, bap, eno, and fib genes. In conclusion, the study revealed varying prevalence rates of adhesion and biofilm-related genes in S. aureus isolates. Genes such as clfB, eno, and icaD were found to be highly prevalent, while bap and bbp were less common. Limited existing data on the prevalence of genes like sasG, bbp, bap, eno, and fib highlights the need for further research to determine their exact prevalence rates. Our results contribute to a better understanding of S. aureus pathogenesis and can facilitate the development of effective strategies for the prevention and treatment of S. aureus infections.
金黄色葡萄球菌是一种多能病原体，能够引发从轻微皮肤感染到危及生命的侵袭性疾病等广泛的感染。金黄色葡萄球菌的致病性归因于其产生多种毒力因子的能力，包括与黏附及生物膜相关的蛋白质。了解这些基因在来自不同来源的金黄色葡萄球菌分离株中的流行情况和分布，对于制定对抗生物膜相关污染的有效策略至关重要。在本研究中，我们进行了一项全面的网络荟萃分析，以评估金黄色葡萄球菌分离株中黏附及生物膜相关基因的流行情况，并研究分离株来源对这些基因出现的影响。我们对多个数据库进行了系统检索，共纳入53项相关研究。确定了金黄色葡萄球菌分离株中黏附及生物膜相关基因的流行情况，其中clfB的流行率最高（p估计值 = 85.4，95%置信区间[CI] 78 - 90.6），其次是eno（p估计值 = 81.1，95% CI 61.7 - 91.9）和icaD（p估计值 = 77，95% CI 68.6 - 83.6）。相反，bap和bbp基因的流行率最低（p估计值分别为6.7和18.7）。网络荟萃分析使我们能够研究金黄色葡萄球菌分离株中黏附及生物膜相关基因的成对共同研究情况。最常共同研究的基因对是icaA - icaD（30次）和fnbA - fnbB（25次）。亚组分析表明，与人类和食品分离株相比，动物分离株中icaC和icaB基因的出现率显著较低（p < 0.05）。值得注意的是，可用于分析sasG、bbp、bap、eno和fib基因的数据有限。总之，该研究揭示了金黄色葡萄球菌分离株中黏附及生物膜相关基因的不同流行率。发现clfB、eno和icaD等基因高度流行，而bap和bbp则较少见。关于sasG、bbp、bap、eno和fib等基因流行率的现有数据有限，这凸显了进一步研究以确定其确切流行率的必要性。我们的结果有助于更好地理解金黄色葡萄球菌的发病机制，并有助于制定预防和治疗金黄色葡萄球菌感染的有效策略。
