Zhu Yanyan, Luo Jingya, Xia Xianzhu, Feng Hao, Zhao Pingsen
Department of Laboratory Medicine, Yuebei People's Hospital Affiliated to Shantou University Medical College, Shaoguan, 512025, China.
Laboratory for Diagnosis of Clinical Microbiology and Infection, Yuebei People's Hospital Affiliated to Shantou University Medical College, Shaoguan, 512025, China.
Heliyon. 2024 Nov 15;10(22):e40281. doi: 10.1016/j.heliyon.2024.e40281. eCollection 2024 Nov 30.
This study aimed to investigate the proportion of neutrophils among leukocytes, in various tissues following intraperitoneal injection of and in mice.
Twelve specific-pathogen free (SPF) male mice, aged eight weeks, were segregated into three groups, each containing four mice. Two of these groups were subjected to intraperitoneal injections of and , both in high concentrations, to establish mouse models of inflammation. The remaining group, which received an intraperitoneal injection of phosphate buffered saline (PBS), served as the control group. Observe the mice every half hour. Then mice were anesthetized, and samples from peripheral blood, liver, and brain tissues were carefully collected nearing death. These samples underwent a digestion process to produce single-cell suspensions. Subsequently, these suspensions were stained with fluorescent antibodies targeting CD45, Ly6G, and CD11b. A flow cytometric analyzer was then employed to enumerate and compare the neutrophil alterations across each group (Fig. 1).
The results indicated a significant variation in the ratio of CD11b Ly6G neutrophils to CD45 leukocytes among the groups. In peripheral blood, the control group showed a neutrophil proportion of approximately 1.44 %, while the and groups exhibited increased proportions of 6.53 % and 3.82 %, respectively. In liver tissue, a marked elevation was observed in the experimental groups, with ratios of 19.20 % and 20.40 % for and , respectively, compared to 1.64 % in the control. In brain tissue, the increments were more modest but noticeable, with the experimental groups showing 2.40 % and 1.11 % in contrast to 0.13 % in the control group.
These findings suggest neutrophils are involved in the response after intraperitoneal injection of and , with marked differences in neutrophil responses in different tissues. This study enhances our understanding of the acute inflammatory response to bacterial infection.
本研究旨在调查小鼠腹腔注射[具体物质1]和[具体物质2]后，不同组织中白细胞中中性粒细胞的比例。
将12只8周龄的无特定病原体（SPF）雄性小鼠分为三组，每组4只。其中两组腹腔注射高浓度的[具体物质1]和[具体物质2]，以建立炎症小鼠模型。其余一组腹腔注射磷酸盐缓冲盐水（PBS），作为对照组。每半小时观察一次小鼠。然后将小鼠麻醉，在濒死时仔细采集外周血、肝脏和脑组织样本。这些样本经过消化过程制成单细胞悬液。随后，用靶向CD45、Ly6G和CD11b的荧光抗体对这些悬液进行染色。然后使用流式细胞分析仪对每组中性粒细胞的变化进行计数和比较（图1）。
结果表明，各组中CD11b Ly6G中性粒细胞与CD45白细胞的比例存在显著差异。在外周血中，对照组的中性粒细胞比例约为1.44%，而[具体物质1]组和[具体物质2]组分别增至6.53%和3.82%。在肝组织中，实验组显著升高，[具体物质1]组和[具体物质2]组的比例分别为19.20%和20.40%，而对照组为1.64%。在脑组织中，增加幅度较小但明显，实验组分别为2.40%和1.11%，而对照组为0.13%。
这些发现表明中性粒细胞参与了腹腔注射[具体物质1]和[具体物质2]后的反应，且不同组织中的中性粒细胞反应存在显著差异。本研究增进了我们对细菌感染急性炎症反应的理解。
