Guo Zhen, Jin Shenghang, Yang Meiying, Fu Luxuan, Ran Yan, Yu Yan, Wang Weizhong
Department of Clinical Laboratory, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China.
Center for Rehabilitation Medicine, Department of Ophthalmology, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, China.
Anal Chim Acta. 2025 Jan 15;1335:343452. doi: 10.1016/j.aca.2024.343452. Epub 2024 Nov 20.
Breast cancer CTCs have recently been recognized as an emerging biomarker for liquid biopsy of breast cancer. In this work, based on two-dimensional (2D) noble metal PtCo@rGO nanozymes and Au@CNTs bioconjugates, a novel electrochemiluminescence (ECL) cytosensor was developed in order to detect breast cancer CTCs (MCF-7) ultrasensitively.
The PtCo@rGO nanozymes possessed large specific surface area and high efficiency peroxidase-like activity, which can be used as nanocarriers to anchor and catalyze luminol ECL emission efficiently. Moreover, the PtCo@rGO nanozymes have fractal nanostructures similar to that of CTCs and can capable of enhancing the adhesion of MCF-7 when assembled together with aptamers containing HS-modified epithelial specific cell adhesion molecules (EpCAM, S1). Importantly, the S1/Au@CNTs bioconjugates loaded on the glassy carbon electrode (GCE) can effectively capture MCF-7 cells. Benefiting from the above-mentioned advantages, the ECL cytosensor constructed for MCF-7 cells detection performed well with a wide linear range (2-1 × 10 cells mL) and a low limit of detection (1 cells mL).
The designed ECL cytosensor could provide a promising platform for CTC-based liquid biopsy and have broad application prospects in breast cancer early diagnosis and prognostic monitoring.
乳腺癌循环肿瘤细胞（CTCs）最近被认为是乳腺癌液体活检的一种新兴生物标志物。在这项工作中，基于二维（2D）贵金属PtCo@rGO纳米酶和Au@CNTs生物共轭物，开发了一种新型电化学发光（ECL）细胞传感器，用于超灵敏检测乳腺癌CTCs（MCF-7）。
PtCo@rGO纳米酶具有大的比表面积和高效的类过氧化物酶活性，可作为纳米载体有效锚定和催化鲁米诺ECL发射。此外，PtCo@rGO纳米酶具有与CTCs相似的分形纳米结构，与含有HS修饰上皮特异性细胞粘附分子（EpCAM，S1）的适配体组装在一起时，能够增强MCF-7的粘附。重要的是，负载在玻碳电极（GCE）上的S1/Au@CNTs生物共轭物能够有效捕获MCF-7细胞。得益于上述优势，构建的用于检测MCF-7细胞的ECL细胞传感器表现良好，具有宽线性范围（2 - 1×10细胞/mL）和低检测限（1细胞/mL）。
所设计的ECL细胞传感器可为基于CTCs的液体活检提供一个有前景的平台，在乳腺癌早期诊断和预后监测中具有广阔的应用前景。
