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通过富含木瓜蛋白酶水解油体增强植物奶的乳脂感。

Enhanced creaminess of plant-based milk via enrichment of papain hydrolyzed oleosomes.

作者信息

Li Biao, Han Chuanwu, Feng Guangxin, Guo Jian, Wan Zhili, Yang Xiaoquan

机构信息

Laboratory of Food Proteins and Colloids, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.

Laboratory of Marin Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong Province 266003, China.

出版信息

Food Res Int. 2024 Dec;198:115322. doi: 10.1016/j.foodres.2024.115322. Epub 2024 Nov 9.

Abstract

There is an increased consumer demand for plant-based milk in substituting dairy milk due to the ethical, health concerns and environmentally-friendly choice. However, perceived creaminess as dominant attributes present a big challenge in consumer acceptance for those milk alternatives. In this study, we developed a novel and easily scalable strategy to enhance the creaminess of soy milk via enrichment of oleosomes. The soybean oleosome creams were extracted and hydrolyzed with papain, resulting in formation of oil droplets with more phospholipid and less protein at the surface, which significantly reduce friction coefficient in the presence of saliva (from 0.15 to 0.03 at a speed around 50 mm/s). Moreover, blending papain-hydrolyzed oleosome creams with raw soy milk enables the creation of a plant-based milk that matches the nutritional profile, lubrication properties, and creaminess of full-fat dairy milk. QCM-D and passive microrheology were employed to characterize hydration of oleosomes into the mucin layer and relevant viscosity change, suggesting that papain hydrolyzed oleosome might decrease friction coefficient via hydration lubrication mechanism. This approach could be applied to enhance the creaminess mouthfeel and nutritional profile of plant-based milk.

摘要

由于伦理、健康问题以及环保考量,消费者对植物基牛奶替代乳制品的需求日益增加。然而,将乳脂感视为主要属性给这些牛奶替代品的消费者接受度带来了巨大挑战。在本研究中,我们开发了一种新颖且易于扩展的策略,通过富集油体来增强豆浆的乳脂感。提取大豆油体乳霜并用木瓜蛋白酶水解,导致表面形成磷脂更多而蛋白质更少的油滴,这在有唾液存在的情况下显著降低了摩擦系数(在约50毫米/秒的速度下从0.15降至0.03)。此外,将木瓜蛋白酶水解的油体乳霜与生豆浆混合能够创造出一种植物基牛奶,其营养成分、润滑特性和乳脂感与全脂乳制品相匹配。采用石英晶体微天平 - 耗散监测技术(QCM - D)和被动微观流变学来表征油体在粘蛋白层中的水合作用以及相关的粘度变化,表明木瓜蛋白酶水解的油体可能通过水合润滑机制降低摩擦系数。这种方法可用于增强植物基牛奶的乳脂感口感和营养成分。

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